Japanese
 NBRP Rat No: 0100 Strain NameMV/Opu Commmon Name: myelin vacuolation rat, mv rat
 Principal Investigator  Mitsuru Kuwamura
 Organization   Osaka Metropolitan University Laboratory of Veterinary Pathology
 Address  1-58, Rinku-Ourai Kita, Izumisano

598-8531 Osaka

 Japan
 Telephone  072-463-5342  Fax:   072-463-5346  kuwamura@vet.osakafu-u.ac.jp
 Inbred Generations    
   
 Coat Color
 Deposition Status
 
 albino (c)
  Embryo      Sperm      Live Animals
 Usage Restrictions  In publishing, a citation of the following literature(s) (*) designated by the DEPOSITOR is requested. 
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability   
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others 
  Control Strains   Reporter gene Strains  
 Gene Atrn: attractin
 Origin The myelin vacuolation (mv) rats showing body tremor were found in an outbred colony of Sprague-Dawley rats at Osaka Osaka Prefecture University in 1999. 
 Strain Characteristics The tremor behavior was exhibited from around 3 weeks of age and inherited as an autosomal recessive trait. The mutant rats had variously sized vacuoles in the neuropil and white matter throughout the central nervous system, especially in the brain stem, cerebellum, and spinal cord. Ultrastructurally these vacuoles mainly consisted of splitting of myelin lamella both in the periaxonal and intermyelinic spaces. No abnormality was found in the number and morphology of oligodendrocytes in MV rats, although the severity and extent of myelin disorder increased with age. Coincident with the myelin abnormalities, there was progressive astrogliosis from 2 weeks. Marked microglial activation was observed exclusively in the gray matter of MV rats from 6 weeks, coincident with severe myelin disruption. Genetic studies revealed 6.914-bp deletion including the whole exon 1 of the attractin (<i>Atrn</i>) gene in MV rats and Northern blot analysis showed no expression of Atrn mRNA in the brain of MV rats. Therefore, the MV rat is a null mutation of the <i>Atrn</i> gene. (Mar 9, 2009) 
 Breeding Conditions Although affected rats are fertile for both sexes, it is recommended to use heterozygous males and females for mating. 
 Genotyping <a href="http://www.anim.med.kyoto-u.ac.jp/nbr/documents/PCR_Gene/Atrn_mv_en.pdf">Genotyping protocol for Atrn_mv</a> 
 References  Izawa T, Yamate J, Franklin RJ, Kuwamura M.
Abnormal myelinogenesis both in the white and gray matter of the attractin-deficient mv rat.
Brain Res. 2010 Feb 2;1312:145-55.

Izawa T, Takenaka S, Ihara H, Kotani T, Yamate J, Franklin RJ, Kuwamura M.
Cellular responses in the spinal cord during development of hypomyelination in the mv rat.
Brain Res. 2008 Feb 21;1195:120-9.


* Tokuda S, Kuramoto T, Serikawa, T.
PCR-based genotyping of the rat Atrn<mv> mutation.
Exp Anim 53(1): 73-76, 2004.

* Kuwamura M, Maeda M, Kuramoto T, Kitada K, Kanehara T, Moriyama M, Nakane Y, Yamate J, Ushijima T, Kotani T, Serikawa T.
The myelin vacuolation (mv) rat with a null mutation in the attractin gene.
Lab Invest. 2002 Oct;82(10):1279-86.