NBRP Rat No: 0140 Strain NameF344/Stm Commmon Name: 
 Principal Investigator  Ryoji Hokao
 Organization   Institute for Animal Reproduction 
 Address  1103, Fukaya, Kasumigaura,

300-0131 Ibaraki

 Telephone  029-897-0631  Fax:  029-897-0633  hokao_ryoji@iar.or.jp
 Inbred Generations   F64+27 (April 2012) 
 Coat Color
 Deposition Status
 albino (a,B,c,h,R)
  Embryo      Sperm      Live Animals
 Usage Restrictions   
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability   
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others 
  Control Strains   Reporter gene Strains  
 Origin F344/Stm rats were derived from a pair of F344/DuCrj rats purchased from Charles River Japan, Inc. and thereafter maintained by brother-sister matings for >23 generations in Saitama Cancer Center Research Institute. 
 Strain Characteristics A parental strain of LEXF/FXLE RI strains (NBRP No.0105-0138).<br />
This strain has a loss-of-function misssense mutation (Gly633Arg) in DIPEPTIDYL PEPTIDASE IV(Dpp4)gene as F344/DuCrlCrlj strain. (July 7, 2016) 
 Breeding Conditions Good breeding performance. Sib mating. 
 References  Yoshihara M, Sato T, Saito D, Ohara O, Kuramoto T, Suyama M.
Application of target capture sequencing of exons and conserved non-coding sequences to 20 inbred rat strains.
Genomics Data. 2016 Dec; 10: 155-157

Taketsuru H, Kaneko T.
In vitro maturation of immature rat oocytes under simple culture conditions and subsequent developmental ability.
J Reprod Dev. 2016 Jul 2. [Epub ahead of print]

Nakanishi S, Serikawa T, Kuramoto T.
Slc:Wistar Outbred Rats Show Close Genetic Similarity with F344 Inbred Rats.
Exp Anim. 64(1):25-29, 2015.

Kaneko T, Mashimo T.
Creating Knockout and Knockin Rodents Using Engineered Endonucleases via Direct Embryo Injection.<br />
Methods Mol Biol. 1239:307-315, 2015.

Yoshimi K, Kaneko T, Voigt B, Mashimo T.
Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR-Cas platform.
Nat Commun. 5:4240, 2014.

Taketsuru H, Kaneko T.
Efficient collection and cryopreservation of embryos in F344 strain inbred rats.
Cryobiology. 2013 Oct;67(2):230-4.

Voigt B, Kuramoto T, Mashimo T, Tsurumi T, Sasaki Y, Hokao R, Serikawa T.
Evaluation of LEXF/FXLE rat recombinant inbred strains for genetic dissection of complex traits.
Physiol Genomics. 2008 Feb 19;32(3):335-42.

Hashimoto R, Voigt B, Ishimaru Y, Hokao R, Chiba S, Serikawa T, Sasa M, Kuramoto T.
Identification of QTLs involved in the development of amygdala kindling in the rat.
Exp Anim. 2013;62(3):181-7.

Shisa, H. et al.
The LEXF:a new set of rat recombinant inbred strains between LE/Stm and F344.
Mamm, Genome.8:324-327(1997).

Lu, L-M., Shisa H. et al.
Propynitrosourea-induced T-lymphomas in LEXF RI strains of rats:genetic analysis.
Br. J.Cancer. 80:855-861(1999)