Japanese
 NBRP Rat No: 0029 Strain Name: KMI/Tky Commmon Name: KMI, Komeda miniature rat Ishikawa
 Principal Investigator  Eiichi Kawamoto
 Organization   Tokyo Medical University Institution: Animal Research Center
 Address  6-1-1 Shinjuku, Shinjuku-ku,

160-8402 Tokyo

  JAPAN
 Telephone  03-3351-6141  Fax:  03-3350-8689  kawamoto@tokyo-med.ac.jp
 Inbred Generations    
   
 Coat Color
 Deposition Status
 
 albino (c)
  Embryo      Sperm      Live Animals
 Usage Restrictions   
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability  Ishikawa Laboratory Animals 
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others 
  Control Strains   Reporter gene Strains  
 Gene Prkg2: protein kinase, cGMP-dependent, type II
 Origin Miniature Rat Ishikawa derived from a breeding colony of Wistar rats at the Ishikawa Animal Laboratory (Saitama). Introduced to Tokyo Medical College. 
 Strain Characteristics Experimental model for pituitary miniaturism with an autosomal recessive mode of inheritance. Body growth in MRI was retarded in 56% and 71% of normal control rats for males and females, respectively. 
 Breeding Conditions Heterozygous female and homozygous male are selected to cross by the phenotype. 
 Genotyping <a href="http://www.anim.med.kyoto-u.ac.jp/nbr/documents/PCR_Gene/prkg2_en.pdf">Genotyping protocol for prkg2</a> 
 References  Chikuda H, Kugimiya F, Hoshi K, Ikeda T, Ogasawara T, Shimoaka T, Kawano H, Kamekura S, Tsuchida A, Yokoi N, Nakamura K, Komeda K, Chung UI, Kawaguchi H
Cyclic GMP-dependent protein kinase II is a molecular switch from proliferation to hypertrophic differentiation of chondrocytes.
Genes Dev 1;18(19):2418-29, 2004.

Serizawa N.
[Initial characterization of a new miniature animal model in the rat: studies on anatomy, pituitary hormones and GH mRNA in miniature rat Ishikawa]
Nippon Naibunpi Gakkai Zasshi 20;69(1):33-45, 1993.

Tsuchida A, Yokoi N, Namae M, Fuse M, Masuyama T, Sasaki M, Kawazu S, Komeda K.
Phenotypic characterization of the Komeda miniature rat Ishikawa, an animal model of dwarfism caused by a mutation in Prkg2.
Comp Med. 58(6):560-7, 2008.