Japanese
NBRP Rat No: 0215
Strain Name
:
W-Tg(Plcb2-WGA,-EGFP)F1Abek
Commmon Name: Wistar-Tg(Plcb2-WGA,-EGFP)F1Abek, Wistar-Tg(PLCb2-WiG)F1
Principal Investigator
Keiko Abe
Organization
Graduate school of Agricultural and Life Sciences ,The University of Tokyo Department of Applied Biological Chemistry
Address
1-1-1, Yayoi, Bunkyo-ku
113-8657 Tokyo
Japan
Telephone
03-5841-5129
Fax: 03-5841-8006
aka7308@mail.ecc.u-tokyo.ac.jp
Inbred Generations
F3
Coat Color
Deposition Status
albino (c)
Embryo
Sperm
Live Animals
Usage Restrictions
The recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
Genetic Status
Inbred
Segregating
Congenic
Consomic
Recombinant
Coisogenic
Spont. Mutant
Transgene
Ind. Mutant
Others
Comercial Availability
Research Category
Diabetes Obesity
Neurobiology
Ophthalmology
Dentistry
Cardio- Hypertension
Oncology
Metabolism
Otorhinology
Immunology
Infectious Disease
Osteology
Internal Medicine
Dermatology
Reproduction
Development
Behavior
Hematology
Urology
Pharmacology
Others
Control Strains
Reporter gene Strains
Gene
Plcb2: phospholipase C, beta 2, GFP: Green Fluorescent Protein, jellyfish, WGA
Origin
Transgene: mouse Plc-b2 promoter, WGA, IRES, EGFP. Background strain: Wistar
Strain Characteristics
WGA and GFP are expressed in almost all PLCb2-positive cells in the taste buds.
Breeding Conditions
Genotyping
primers: 5'-AACCTCTCCAAAGAGGACACTTGAC-3' and 5'-CGTAAGGGCCATGGTGCTCATCATCTTTCT-3'. PCR condition: Ex Taq (TaKaRa) 94℃ 60" (94℃ 20", 55℃ 30", 72℃ 60") x 30, 72℃ 60"
References
発表論文なし