The National BioResource Project for the Rat in Japan

Strain information

Back to the list 202 / 882
NBRP Rat No: 0214
Strain name: W-Tg(Plcb2-WGA,-EGFP)M1Abek
Commmon Name: Wistar-Tg(Plcb2-WGA,-EGFP)M1Abek, Wistar-Tg(PLCb2-WiG)M1
Rat Genome Database
Principal Investigator:
Keiko AbeGraduate school of Agricultural and Life Sciences ,The University of Tokyo
Preservation Status:
Coat Color
albino (c)
Inbred Generations
F2
Usage Restrictions
The recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
Genetic Status
Comercial Availability
Research Category
Gene Affected
Plcb2: phospholipase C, beta 2, GFP: Green Fluorescent Protein, jellyfish, WGA
Origin
Transgene: mouse Plc-b2 promoter, WGA, IRES, EGFP. Background strain: Wistar
Strain characteristics
WGA and GFP are expressed in almost all PLCb2-positive cells in the taste buds.
Breeding Conditions
Genotyping
primers: 5'-AACCTCTCCAAAGAGGACACTTGAC-3' and 5'-CGTAAGGGCCATGGTGCTCATCATCTTTCT-3'. PCR condition: Ex Taq (TaKaRa)　94℃ 60" (94℃ 20", 55℃ 30", 72℃ 60") x 30, 72℃ 60"
References
発表論文なし
Additional strain information (Japanese only)