NBRP Rat No: 0200 Strain NameBUF/Mna Commmon Name: 
 Principal Investigator  Mutsushi Matsuyama
 Organization   Fujita Health University School of Medicine Department of Virology and Parasitology
 Address  1-98 Dengakugakubo, Kutsukake-cho, Toyoake-shi

420-1192 Aichi

 Telephone  0562-93-2486  Fax:  0562-93-4008  mmatsuya@fujita-hu.ac.jp
 Inbred Generations   F101+21 (April 2012) 
 Coat Color
 Deposition Status
 albino (c)
  Embryo      Sperm      Live Animals
 Usage Restrictions  In publishing, a citation of the following literature(s) designated by the DEPOSITOR is requested. 
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability   
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others 
  Control Strains   Reporter gene Strains  
 Gene Actr3: ARP3 actin-related protein 3 homolog (yeast)
 Origin BUF/Mna rats were established from sib mating of Buffalo rat colony transferred from Biological Associates in America to Department of Pathology, Nagoya City University in 1964. At F18 generation, the rats showed thymoma 100% and were named as BUF/Mna in 1977 (Matsuyama, 1977). Buffalo rat. (Oct 12, 2010) 
 Strain Characteristics BUF/Mna rats develop spontaneous thymoma, muscle atrophy and nephropathy.<br />
BUN/Mna starts to develop thymoma at 9 months of age (Matsuyama, 1988). The thymoma was defined as epithelial cell tumors by transplant experiments using ACI rats (Taguchi, 1992). A linkage study revealed that thymoma susceptibility was determined by an autosomal dominant locus <i>Tsr1</i> (thymoma susceptible locus of rat 1) and it was located between <i>D7Wox16</i> and <i>D7Rat21</i> on chromosome 7 (Oyabu, 1999; Higo, 2000). <br />
At pre-thymoma age, BUF/Mna rats have extremely large thymuses mainly in cortex. A linkage analysis showed two polymeric autosomal loci, <i>Ten1</i> and <i>Ten2</i>, regulating thymus enlargement in BUF/Mna and they were mapped on chromosome 1 and 13, respectively (Murakumo, 1996; Ye, 2000). <br />
BUF rats were susceptible to thymic lymphomas (Ogui, 1989) but resistant to MNNG-induced stomach carcinogenesis (Ohgaki, 1983). BUF rats spontaneously develop progressing atrophy of fast-twitch type muscle (Kato, 1982) and proteinuria associated with focal and segmental glomerulosclerosis (Nakamura, 1986). For those phenotypes, one or more QTL genes were mapped (Ogui, 1995; Amo, 1997; Murayama, 1998; Ushijima, 2000). (Oct 12, 2010) 
 Breeding Conditions Sib mating. 
 References  Matsuyama M, Nagayo T
Development of thymoma and myasthenia in Buffalo/Mna rats.
Proc Jpn Cancer Assoc 36th Annu Meet 30, 1977.

Kato F, Watanabe M.
Motor dysfunction in spontaneous thymoma rats, Buffalo/Mna.
J Pharmacobiodyn. 1982 Sep;5(9):727-33.

Ohgaki H, Kawachi T, Matsukura N, Morino K, Miyamoto M, Sugimura T.
Genetic control of susceptibility of rats to gastric carcinoma.
Cancer Res. 1983 Aug;43(8):3663-7.

Nakamura T, Oite T, Shimizu F, Matsuyama M, Kazama T, Koda Y, Arakawa M.
Sclerotic lesions in the glomeruli of Buffalo/Mna rats.
Nephron. 1986;43(1):50-5.

Ogiu T, Fukami H, Matsuyama M, Maekawa A.
Thymic lymphomas induced by N-propyl-N-nitrosourea (PNU) in the BUF/Mna rat, an inbred strain with a high incidence of spontaneous thymoma.
Acta Pathol Jpn. 1989 Nov;39(11):706-11.

Taguchi O, Kontani K, Ikeda H, Matsuyama M.
An intrinsic thymic epithelial abnormality is responsible for the spontaneous development of predominantly lymphocytic thymomas in BUF/Mna rats.
Jpn J Cancer Res. 1992 Nov;83(11):1166-71.

Ogiu T, Fukami H, Nishimura M, Matsuyama M.
Genetic regulation of development of thymic lymphomas induced by N-propyl-N-nitrosourea in the rat.
Jpn J Cancer Res. 1995 Jul;86(7):638-44.

Murakumo Y, Takahashi M, Sharma N, Arakawa A, Saito M, Amo H, Matsuyama M.
Mapping of two genetic loci, Ten-1 and Ten-2, associated with thymus enlargement in BUF/Mna rats.
Mamm Genome. 1996 Jul;7(7):505-8.

藤田学園医学会誌 1996 20(2):191-7.

Sharma N, Ohyabu A, Murakumo Y, Takahashi M, Saito M, Amo H, Murayama S, Ohno K, Oda S, Matsuyama M.
Genetic mapping of genes regulating the thymus size in back-cross rats between the laboratory BUF/Mna strain and the MITE strain derived from the wild rat, Rattus norvegicus.
Pathol Int. 1997 Jul;47(7):436-41.

Amo H, Saito M, Nagao S, Yagiu S, Takahashi H, Morita H, Morita TM, Matsuyama M.
Genetic regulation of slowly progressing mild muscle atrophy in fast-twitch muscles of BUF/Mna rats.
Muscle Nerve. 1997 Oct;20(10):1258-63

Murayama S, Yagyu S, Higo K, Ye C, Mizuno T, Oyabu A, Ito M, Morita H, Maeda K, Serikawa T, Matsuyama M.
A genetic locus susceptible to the overt proteinuria in BUF/Mna rat.
Mamm Genome. 1998 Nov;9(11):886-8

Oyabu A, Higo K, Ye C, Amo H, Saito M, Yagyu S, Morita H, Maeda K, Serikawa T, Takahashi M, Matsuyama M.
Genetic mapping of the thymoma susceptible locus, Tsr1, in BUF/Mna rats.
J Natl Cancer Inst. 1999 Feb 3;91(3):279-82

Ushijima T, Yamamoto M, Suzui M, Kuramoto T, Yoshida Y, Nomoto T, Tatematsu M, Sugimura T, Nagao M.
Chromosomal mapping of genes controlling development, histological grade, depth of invasion, and size of rat stomach carcinomas.
Cancer Res. 2000 Feb 15;60(4):1092-6.

Ye C, Murakumo Y, Higo K, Matsuyama M.
Fine mapping of thymus enlargement gene 1 (Ten1) in BUF/Mna rats.
Pathol Int. 2000 Mar;50(3):185-90.

Higo K, Ushijima T, Oyabu A, Ye C, Yagyu S, Takahashi H, Matsuyama M.Nakamura T, Oite T, Shimizu F, Generation of a polymorphic marker linked to thymoma susceptibility gene of rat 1 by genetically-directed representational difference analysis.
Exp Anim. 2000 Jul;49(3):189-95.

Akiyama K et al
Actin-related protein 3 (Arp3) is mutated in proteinuric BUF/Mna rats
Mamm Genome. 2008 Jan;19(1):41-50.

Matsuyama M, Haneda C, Kato K.
Congenic strain differences of renal malformations in ACI/Mna rats by introgression of the chromosomal region of BUF/Mna rats containing Pur1.
Nagoya J Med Sci. 75(3-4):251-254, 2013.

Matsuyama M, Kato K.
Refusal of Food Ingestion of Old Male BUF/Mna Rats in Metabolic Cages.
Research in Psychology and Behavioral Sciences 3, no. 1 (2015): 5-6.

Clement LC, Mac&eacute; C, Avila-Casado C, Joles JA3, Kersten S, Chugh SS.
Circulating Angiopoietin-like 4 links proteinuria with hypertriglyceridemia in nephrotic syndrome.
Nat Med. 2014 Jan;20(1):37-46.

Yoshihara M, Sato T, Saito D, Ohara O, Kuramoto T, Suyama M.
Application of target capture sequencing of exons and conserved non-coding sequences to 20 inbred rat strains.
Genomics Data. 2016 Dec; 10: 155-157