Japanese
 NBRP Rat No: 0008 Strain Name: IS/Kyo Commmon Name: Ishibashi rat, ISR
 Principal Investigator  Tadao Serikawa
 Organization   Graduate School of Medicine, Kyoto University Institute of Laboratory Animals
 Address  Yoshidakonoe-cho, Sakyo-ku

606-8501 Kyoto

  JAPAN
 Telephone  075-753-4360  Fax:  075-753-4409  serikawa@anim.med.kyoto-u.ac.jp
 Inbred Generations   F20+68 (March 2012) 
   
 Coat Color
 Deposition Status
 
 agouti (A,B,C)
  Embryo      Sperm      Live Animals
 Usage Restrictions  The recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
For a commercial use of this resource, a new contract must be concluded between the depositor and the recipient. 
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability   
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others 
  Control Strains   Reporter gene Strains  
 Gene
 Origin Ishibashi rat derived from a cross between a wild male and a Wistar female, with sib mating since 1975 at Azabu University, transferred to Kyoto University in 1978 (IS/Kyo). 
 Strain Characteristics Malformations of the thoracicolumbar vertebrae leading to kyphoscoliosis with restricted spinal canals and compressed spinal cords. The malformations occur from the 12th. thoracic to the 6th. lumbar vertebrae. Up to 7 vertebrae are affected, and some degree of abnormality occurs in 90% of individuals. The expression levels of Hox10 and Hox11 paralogs, which regulate the formation of lumbar and sacral vertebrae, is extremely low in lumbo-sacral transitional areas of ISR compared with those of normal rats (Seki, 2008). The IS rat shows the low plasma alkaline phosphatase (ALP) activity but there is no relations between the ALP activity and the vertebral anomalies (Maekawa, 1982). (Jun 4, 2009) 
 Breeding Conditions Good breeding performance. Sib mating. 
 Genotyping  
 References  Sato T, Takahashi I, Watanabe Y, Yokoyama D, Shimokawa N.
Congenital kyphoscoliosis: Analysis of vertebral abnormalities using model animals (Review).
Exp Ther Med. 2024 Sep 4;28(5):416.

Takahashi I, Watanabe Y, Sonoda H, Tsunoda D, Amano I, Koibuchi N, Iizuka H, Shimokawa N.
Calcium sensing and signaling are impaired in the lumbar spine of a rat model of congenital kyphosis.
Eur Spine J. 2023 Oct;32(10):3403-3412.


Ishiwata S, Iizuka H, Sonoda H, Tsunoda D, Tajika Y, Chikuda H, Koibuchi N, Shimokawa N.
Upregulated miR-224-5p suppresses osteoblast differentiation by increasing the expression of Pai-1 in the lumbar spine of a rat model of congenital kyphoscoliosis.
Mol Cell Biochem. 2020 Dec;475(1-2):53-62.

Sonoda H, Iizuka H, Ishiwata S, Tsunoda D, Abe M, Takagishi K, Chikuda H, Koibuchi N, Shimokawa N.
The retinol-retinoic acid metabolic pathway is impaired in the lumbar spine of a rat model of congenital kyphoscoliosis.
J Cell Biochem. 2019 Sep;120(9):15007-15017.

Yoshihara M, Sato T, Saito D, Ohara O, Kuramoto T, Suyama M.
Application of target capture sequencing of exons and conserved non-coding sequences to 20 inbred rat strains.
Genomics Data. 2016 Dec; 10: 155-157

Tsunoda D, Iizuka H, Ichinose T, Iizuka Y, Mieda T, Shimokawa N, Takagishi K, Koibuchi N.
The Trk family of neurotrophin receptors is downregulated in the lumbar spines of rats with congenital kyphoscoliosis.
Mol Cell Biochem. 2015 Nov 7. [Epub ahead of print]

Takano M, Ogawa E, Saitou T, Yamaguchi Y, Asano Y, Serikawa T, Kuramoto T.
Morphological features of adult rats of IS/Kyo and IS-Tlk/Kyo strains with lumbar and caudal vertebral anomalies.
Exp Anim. 2014;63(3):269-75.

Kuramoto T, Nakanishi S, Ochiai M, Nakagama H, Voigt B, Serikawa T.
Origins of Albino and Hooded Rats: Implications from Molecular Genetic Analysis across Modern Laboratory Rat Strains.
PLoS One. 2012;7(8):e43059.

Torigoe D, Ichii O, Dang R, Ohnaka T, Okano S, Sasaki N, Kon Y, Agui T.
High-resolution linkage mapping of the rat hooded locus.
J Vet Med Sci. 2011 May;73(5):707-10.

Seki T, Shimokawa N, Iizuka H, Takagishi K, Koibuchi N.
Abnormalities of vertebral formation and Hox expression in congenital kyphoscoliotic rats.
Mol Cell Biochem. 312(1-2):193-9, 2008.

Ishibashi M.
Congenital vertebral malformation (Ishibashi rats).
In Handbook on Animal Models of Human Diseases, pp. 430-434, Kawamata J. and　Matushita H. (edit.) Ishiyaku Shuppan Ltd. Tokyo. 1979.

Maekawa R, Yamada J, Nikaido H.
Genetical studies of low plasma alkaline phosphatase (ALP) activity in the IS　strain of rats.
Jikken Dobutsu. 31:13-19, 1982.

Yamada J, Nikaido H, Moritake S, Maekawa R.
Genetic analyses of the vertebral anomalies of the IS strain of rat and the　development of a BN congenic line with the anomalies.
Lab Anim. 16: 40-47, 1982.

Moritake S, Yamamuro T, Yamada J, Watanabe H.
Progression of congenital kyphosis in Ishibashi rats.
Acta Orthop Scand. 54: 841-846, 1983.