Japanese
 NBRP Rat No: 0269 Strain Name: WF.DA-(D19Wox8-D19Wox7)/Kop Commmon Name: WF.DA-<i>Tcas5</i>/Kop, WF.DA-<i>Tscc1</i>, WDTscc1
 Principal Investigator  Ichiro Semba
 Organization   Kagoshima University Department of Oral Pathology, Filed of Oncology, Graduate School of Medical and Dental Sciences
 Address  8-35-1, Sakuragaoka

890-8544 Kagoshima

  Japan
 Telephone  099-275-6142  Fax:  099-275-6148  semba@dent.kagoshima-u.ac.jp
 Inbred Generations   N5F22 
   
 Coat Color
 Deposition Status
 
 albino (c)
  Embryo      Sperm      Live Animals
 Usage Restrictions  The users should offer a collaboration study plan to Department of Oral Pathology, Kagoshima University Graduate School of Medical and Dental Sciences. 
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability   
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others 
  Control Strains   Reporter gene Strains  
 Gene Tscc1 (Tongue tumor susceptibility QTL 1, alias Tcas5)
 Origin DA rats are highly susceptible to 4NQO-induced tongue carcinoma, whereas WF rats are barely susceptible (Kitano, 1992). To establish this congenic strain, QTL region <i>Tscc1</i> (Tongue squamous cell carcinoma 1, alias <i>Tcas5</i> RGD: 724565) was introgressed from DA rats to WF rats (Tanuma, 1998; Tanuma, 2001). (Aug 20, 2010) 
 Strain Characteristics The numbers and sizes of 4NQO-induced tongue cancer in this congenic rat showed l significantly higher than those in WF rats (Hirano, 2006). In search of the candidate genes for <i>Tscc1</i>, one SNP was found in the promoter region of the putative candidate gene <i>Nqo1</i> (NAD(P)H dehydrogenase, quinone 1, NCBI ID: 24314) (Hirano, 2006). The expression level of <i>Nqo1</i> mRNA and the enzyme activity were several-folds higher in DA and this congenic rat than in WF. (Aug 20, 2010) 
 Breeding Conditions Sib mating. 
 Genotyping  
 References  Kitano M, Hatano H, Shisa H.
Strain difference of susceptibility to 4-nitroquinoline 1-oxide-induced tongue carcinoma in rats.
Jpn J Cancer Res. 1992 Aug;83(8):843-50.

Tanuma J, Shisa H, Hiai H, Higashi S, Yamada Y, Kamoto T, Hirayama Y, Matsuuchi H, Kitano M.
Quantitative trait loci affecting 4-nitroquinoline 1-oxide-induced tongue carcinogenesis in the rat.
Cancer Res. 15;58(8):1660-4, 1998.

Tanuma JI, Fujii K, Hirano M, Matsuuchi H, Shisa H, Hiai H, Kitano M.
Five quantitative trait loci affecting 4-nitroquinoline 1-oxide-induced tongue cancer in the rat.
Jpn J Cancer Res. 2001 Jun;92(6):610-6.

Hirano M, Tanuma J, Hirayama Y, Ohyama M, Semba I, Wakusawa S, Shisa H, Hiai H, Kitano M.
A speed congenic rat strain bearing the tongue cancer susceptibility locus Tscc1 from Dark-Agouti rats.
Cancer Lett. 2006 Jan 18;231(2):185-91.