Japanese
 NBRP Rat No: 0489 Strain Name: F344-Tg(CAG-EGFP)Ncco Commmon Name: F344/DuCrlClrj-Tg(CAG-EGFP)Ncco, F344/DuCrlCrlj-<i>TgN(CAG-EGFP)Ncco</i>, F344-EGFP rat
 Principal Investigator  Takahiro Ochiya
 Organization   Tokyo Medical University Institute of Medical Science  Department of Molecular and Cellular Medicine
 Address   6-7-1 Nishi-Shinjuku, Shinjyuku-ku,

160-0023  Tokyo

 Japan
 Telephone  03-3342-6111  Fax:    tochiya@tokyo-med.ac.jp
 Inbred Generations   F4+14 (March 2012) 
   
 Coat Color
 Deposition Status
 
 albino (c)
  Embryo      Sperm      Live Animals
 Usage Restrictions  The recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing, an acknowledgment to the DEPOSITOR is requested.
For a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE, the recipient shall acquire the consent from the DEPOSITOR.
 
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability   
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others Regeneration Medicine
  Control Strains   Reporter gene Strains  
 Gene GFP: Green Fluorescent Protein, jellyfish
 Origin Transgenic rat: CAG promoter, Enhanced Green Fluorescent Protein gene, microinjection method 
 Strain Characteristics Fluorescence is detected from almost whole tissues; skin, brain, heart, lung, spleen, liver, kidney, testis, sperm, uterus, muscle. 
 Breeding Conditions This strain is maintained by natural mating of Tg rats showing fluorescence with F344Du/Crj. 
 Genotyping Tg rats are selected by checking green fluorescence.
<a href="http://www.anim.med.kyoto-u.ac.jp/nbr/documents/PCR_Gene/transgenic Rat protocol of genotyping(EGFP)170412.pdf">Genotyping protocol for EGFP</a>
 
 References  Watanabe H, Ochiya T, Ueda S, Kominami Y, Gon R, Nishiki M, Hayashi M, Sasaki A, Shiraishi M, Kashimoto N, Myojin Y, Kamiya K.
Differentiation of a hepatic phenotype after heterotropic transplantation of heart, kidney, brain, and skin tissues into liver in F344 rats.
Biochem Biophys Res Commun. 2007 Mar 23;354(4):841-5.