Japanese
 NBRP Rat No: 0352 Strain Name: SD-Tg(Nes/Hspa1b-EGFP)Okn Commmon Name: SD-<i>Tg(Nes-Hspa1b-EGFP)Okn</i>, Nestin-EGFP
 Principal Investigator  Hideyuki Okano
 Organization   Keio University School of Medicine, Department of Physiology
 Address  35 Shinano-cho, Shinjyuku-ku

160-8582 Tokyo

  Japan
 Telephone  03-5363-3747  Fax:  03-3357-5445  hidokano@sc.itc.keio.ac.jp
 Inbred Generations   N3 (April 2012) 
   
 Coat Color
 Deposition Status
 
 albino (c)
  Embryo      Sperm      Live Animals
 Usage Restrictions   
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability   
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others 
  Control Strains   Reporter gene Strains  
 Gene Nes (nestin）, Hspa1b (heat shock 70kD protein 1B), GFP (Green Fluorescent Protein, jellyfish)
 Origin This transgenic strain was established at Slc in 1999 and transferred to Keio University in 2000. 
 Strain Characteristics This strain expresses green fluorescent protein (GFP) neural-specific driven by Nestin enhancer and Hspa1b promoter.  
 Breeding Conditions Maintained in hemizygous condition. Usually, maintained by crossing of male transgenic rats and female wild-type rats. 
 Genotyping Transgenic rats were identified by Southern blot analysis or by PCR using tail DNA and primers specific for the GFP sequence. 
 References  Sawamoto K, Nakao N, Kakishita K, Ogawa Y, Toyama Y, Yamamoto A, Yamaguchi M, Mori K, Goldman SA, Itakura T, Okano H.
Generation of dopaminergic neurons in the adult brain from mesencephalic precursor cells labeled with a nestin-GFP transgene.
J Neurosci. 2001 Jun 1;21(11):3895-903.