| Strain Characteristics |
This mutant rat shows beige coat color, giant granule cells in various tissues, and prolonged bleeding time. This mutant strain is a disease model for human Chediak-Higashi syndrome (CHS). This mutation is a deletion of about 20 kb containing exons 28, 29 and 30, which constitute the 578 bp sequence missing in the <i>Lyst</i> cDNA on rat chromosome 17. This mutant gene is allelic to <i>Lyst<sup>be</sup></i> (DA-beige rat) but the putative deletion break points in L1 elements were different from it (Mori, 2003). The nested PCR-RFLP analysis is useful to discriminate <i>Lyst<sup>be-Kyo</sup></i> from <i>Lyst<sup>be</sup></i> (Masui, 2004). (May 21, 2009) |
| References |
山崎賢一、中西聡、北田一博、芹川忠夫、西川哲、並木千晶、浜田修一、橋本敦<br />
ACI/N系統に出現した新たな淡毛色ラットの解析<br />
第68回関西動物実験研究会要旨<br /><br />
Mori M, Yamasaki K, Nakanishi S, Kitada K, Higuchi K, Namiki C, Hamada S, Serikawa T. <br />
A new beige mutant rat ACI/NKyo-Lyst<bg-Kyo><br />
Exp. Anim. 52(1), 31-36, 2003.<br /><br />
Masui N, Mori M, Nishikawa T, Takagi Y, Asai H, Yanabe M, Yamasaki K, Serikawa T, Sato K.<br />
An allele-specific genotyping method for rat lyst (lysosomal trafficking regulator) gene.<br />
Exp Anim. 2004 53(1):77-80.<br /> |