NBRP Rat No: 0002 Strain NameACI-Lystbg-Kyo/Kyo Commmon Name: ACI/NKyo-<i>Lyst<sup>bg-Kyo</sup></i>/Kyo, ACI/NKyo-<i>Lyst<sup>bg-Kyo</sup></i>, ACI/N-bg
 Principal Investigator  Tadao Serikawa
 Organization   Graduate School of Medicine, Kyoto University Institute of Laboratory Animals
 Address  Yoshidakonoe-cho, Sakyo-ku

606-8501 Kyoto

 Telephone  075-753-4360  Fax:  075-753-4409  serikawa@anim.med.kyoto-u.ac.jp
 Inbred Generations   F35+F27 (May 2009) 
 Coat Color
 Deposition Status
 beige (A,B,hi,bg)
  Embryo      Sperm      Live Animals
 Usage Restrictions   
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability   
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others 
  Control Strains   Reporter gene Strains  
 Gene Lyst: lysosomal trafficking regulator
 Origin Spontaneous mutation from ACI/NKyo inbred at Kyoto University in 1999. 
 Strain Characteristics This mutant rat shows beige coat color, giant granule cells in various tissues, and prolonged bleeding time. This mutant strain is a disease model for human Chediak-Higashi syndrome (CHS). This mutation is a deletion of about 20 kb containing exons 28, 29 and 30, which constitute the 578 bp sequence missing in the <i>Lyst</i> cDNA on rat chromosome 17. This mutant gene is allelic to <i>Lyst<sup>be</sup></i> (DA-beige rat) but the putative deletion break points in L1 elements were different from it (Mori, 2003). The nested PCR-RFLP analysis is useful to discriminate <i>Lyst<sup>be-Kyo</sup></i> from <i>Lyst<sup>be</sup></i> (Masui, 2004). (May 21, 2009) 
 Breeding Conditions Maintained by crossing of homozygoutes. Litter size is small. 
 References  山崎賢一、中西聡、北田一博、芹川忠夫、西川哲、並木千晶、浜田修一、橋本敦<br />
ACI/N系統に出現した新たな淡毛色ラットの解析<br />
第68回関西動物実験研究会要旨<br /><br />

Mori M, Yamasaki K, Nakanishi S, Kitada K, Higuchi K, Namiki C, Hamada S, Serikawa T. <br />
A new beige mutant rat ACI/NKyo-Lyst<bg-Kyo><br />
Exp. Anim. 52(1), 31-36, 2003.<br /><br />

Masui N, Mori M, Nishikawa T, Takagi Y, Asai H, Yanabe M, Yamasaki K, Serikawa T, Sato K.<br />
An allele-specific genotyping method for rat lyst (lysosomal trafficking regulator) gene.<br />
Exp Anim. 2004 53(1):77-80.<br />