Japanese
 NBRP Rat No: 0470 Strain Name: W-Tg(CAG-EGFP)3Ys Commmon Name: Wistar-Tg(CAG-EGFP)3Ys, Wistar-<i>TgN(CAG-EGFP)3Ys</i>, Wistar GFP
 Principal Investigator  Masatsugu Ueda
 Organization   PhoenixBio Co., Ltd. (YS Institute, Inc.)
 Address  1198-4, Iwaso, Utsunomiya

321-0973 Tochigi

  Japan
 Telephone  028-683-1153  Fax:  028-664-2410  ystg@phoenixbio.co.jp
 Inbred Generations   F14N6 (April 2012) 
   
 Coat Color
 Deposition Status
 
 albino (c)
  Embryo      Sperm      Live Animals
 Usage Restrictions  In publishing, a citation of the following literature(s) designated by the DEPOSITOR is requested. (see references) 
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability   
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others 
  Control Strains   Reporter gene Strains  
 Gene GFP: Green Fluorescent Protein, jellyfish
 Origin GFP: Green Fluorescent Protein, jellyfish 
 Strain Characteristics This transgenic strain contains the enhanced green fluorescent protein (EGFP) gene ubiquitously driven by CAG promoter. 
 Breeding Conditions Tg X CrlJ:WI 
 Genotyping <a href="http://www.anim.med.kyoto-u.ac.jp/nbr/documents/PCR_Gene/transgenic Rat protocol of genotyping(EGFP)170412.pdf">Genotyping protocol for EGFP</a>
 
 References  Morita K, Ihashi S, Okamura E, Goto K, Yoshihara T, Honda A, Ema M, Asano M.
Highly efficient production of transgenic rats with long DNA insertions using piggyBac transposase mRNA and piezo-assisted microinjection.
PLoS One. 2026 Feb 17;21(2):e0339406.

*Hakamata Y, Tahara K, Uchida H, Sakuma Y, Nakamura M, Kume A, Murakami T, Takahashi M, Takahashi R, Hirabayashi M, Ueda M, Miyoshi I, Kasai N, Kobayashi E.
Green fluorescent protein-transgenic rat: a tool for organ transplantation research.
Biochem Biophys Res Commun. 31;286(4):779-85, 2001.

*Hirabayash M, Kato M, Aoto T, Sekimoto A, Ueda M, Miyoshi I, Kasai N, Hochi S.
Offspring derived from intracytoplasmic injection of transgenic rat sperm.
Transgenic Res. 11(2):221-8, 2002.