Japanese
 NBRP Rat No: Strain Name: MPR/Iar Commmon Name: MPR-<i>Arsb<sup>abd</sup></i>/Iar, MPR
 Principal Investigator  Tetsuo Kunieda
 Organization   Okayama University Graduate School of Natural and Technology Applied Animal Genetics
 Address  3-1-1 Tsushima-naka Okayama

700-8530  Okayama

  Japan
 Telephone  086-251-8314  Fax:  086-251-8388  tkunieda@cc.okayama-u.ac.jp
 Inbred Generations   F? 
   
 Coat Color
 Deposition Status
 
 albino (c)
  Embryo      Sperm      Live Animals
 Usage Restrictions  The recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT must agree on collaborative research with the DEPOSITOR. Any form of publications of data resulting from the use of the BIOLOGICAL RESOUCE require co-authorship.  
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability   
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others 
  Control Strains   Reporter gene Strains  
 Gene Arsb: arylsulfatase B
 Origin This mutant was found as a rat exhibits facial and skeletal dysmorphism in the Ishibashi hairless rat strain at Imamichi Institute for Animal Reproduction in 1988 (Yoshida, 1993). (Jun 3, 2010) 
 Strain Characteristics MPR rats have smaller bodies, shorter limbs, facial dysmorphia, dysostosis multiplex, and increased urinary excretion of glucosaminoglycans (GAGs) and these features become evident after 3 weeks of age (Yoshida, 1993). Biochemical analyses demonstrated that the excreted CAG was dermantan sulfate and the activity of hepatic arylsulfatase B was lower than that of normal rats.
MPR rats have a 1-bp insertion (507insC) which resulting in a frame shift mutation in the arylsulfatase B (<i>Arsb</i>) gene on rat chromosome 2 (Kunieda, 1995). The MPR is a disease model for mucopolysaccharidosis VI. (Jun 3, 2010) 
 Breeding Conditions Homozygous <i>Arsb</i> rats are difficult to breed and are therefore maintained by crossing heterozygous individuals (segregating inbred strain). 
 Genotyping <a href="http://www.anim.med.kyoto-u.ac.jp/nbr/documents/PCR_Gene/Arsb_en.pdf">Genotyping protocol for Arsb</a> 
 References  Yoshida M, Noguchi J, Ikadai H, Takahashi M, Nagase S.
Arylsulfatase B-deficient mucopolysaccharidosis in rats.
J Clin Invest. 91(3):1099-104, 1993.

Kunieda T, Simonaro CM, Yoshida M, Ikadai H, Levan G, Desnick RJ, Schuchman EH.
Mucopolysaccharidosis type VI in rats: isolation of cDNAs encoding arylsulfatase B, chromosomal localization of the gene, and identification of the mutation.
Genomics. 10;29(3):582-7, 1995.