Japanese
 NBRP Rat No: 0478 Strain Name: DA.Cg-Foxn1rnu Lystbg/Slc Commmon Name: DA-<i>Foxn1<sup>rnu</sup> Lyst<sup>bg</sup></i>/Slc, DA/Slc-<i>Foxn1<sup>rnu</sup> Lyst<sup>bg</sup></i>, DA/Slc-bg rnu
 Principal Investigator  Norio Masui
 Organization   Japan SLC,Inc. 
 Address  3-5-1, Aoi-Higashi, Naka-ku, Hamamatsu city

433-8114 Shizuoka

  Japan
 Telephone  053-437-5348  Fax:  053-436-4320  n-masui@jslc.co.jp
 Inbred Generations   ?＋N16 
   
 Coat Color
 Deposition Status
 
 dark agouti （heterozygous）、　hairless （homozygous）
  Embryo      Sperm      Live Animals
 Usage Restrictions   
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability   
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others 
  Control Strains   Reporter gene Strains  
 Gene Foxn1: forkhead box N1 ,Lyst: lysosomal trafficking regulator
 Origin Three strains of rats (DA/Slc, DA-Foxn1rnu and DA/Ham-Lystbg) were selected to establish this congenic strain. DA/Ham-Lystbg strain was derived from the beige mutation found in the inbred colony of the DA/Ham at Hamamatsu University of Medicine in 1985. DA-Foxn1rnu strain was produced by cross-intercross method with five backcross generation between an athymic nude rat and the DA/Slc strain. By crossing these three strains, DA-<i>Foxn1<sup>rnu</sup> Lyst<sup>bg</sup></i> double mutant rat strain was established (Masui, 2004). (May 21, 2009) 
 Strain Characteristics This mutant strain is an athymic beige-nude rat and shows a bleeding tendency and impaired NK cell activity. (May 21, 2009) 
 Breeding Conditions Both male and female of heterozygous for <i>Foxn1<sup>rnu</sup></i> <i>Lyst<sup>bg</sup></i> double mutant are fertile and it is also possible to cross between <i>Foxn1<sup>rnu</sup></i>/+ and <i>Lyst<sup>bg</sup></i>/<i>Lyst<sup>bg</sup></i> rats. (May 21, 2009) 
 Genotyping <a href="http://www.anim.med.kyoto-u.ac.jp/nbr/documents/PCR_Gene/Foxn1_rnu.pdf">Genotyping protocol for Foxn1</a> 
 References  Masui N, Nishikawa T, Takagi Y, Mori M, Suzuki T, Sato K.
The rat lysosomal trafficking regulator (Lyst) gene is mapped on the telomeric region of chromosome 17.
Exp Anim. 2003 52(1):89-91.

Masui N, Mori M, Nishikawa T, Takagi Y, Asai H, Yanabe M, Yamasaki K, Serikawa T, Sato K.
An allele-specific genotyping method for rat lyst (lysosomal trafficking regulator) gene.
Exp Anim. 2004 53(1):77-80.

Masui N, Nishikawa T, Takagi Y, Kimura H, Mori M, Yokose S, Asai H, Gonda T, Yanabe M, Sato K.
Establishment of a set of combined immunodeficient DA/Slc-Foxn1(rnu) Lyst(bg) congenic rat strains.
Exp Anim. 2004 53(5):399-407.