Japanese
 NBRP Rat No: 0491 Strain NameWIC-Tg(Wap-GH1)2Mni Commmon Name: WistarImamichi-Tg(Wap-GH1)2Mni, WistarImamichi-<i>Tg(mWAP-hGH)2Mni</i>,&nbsp;WAP/hGH-transgenic rat (low line)
 Principal Investigator  Masugi Nishihara
 Organization   Agricultural and Life Sciences ,The University of Tokyo Laboratry of verterinary physiology
 Address  1-1-1 Bunkyo-ku, Yayoi

113-8657  Tokyo

 Japan
 Telephone  03-5841-5386  Fax:  03-5841-8017  amnishi@mail.ecc.u-tokyo.ac.jp
 Inbred Generations    
   
 Coat Color
 Deposition Status
 
 albino (c)
  Embryo      Sperm      Live Animals
 Usage Restrictions  In publishing, a citation of the following literature(s) designated by the DEPOSITOR is requested. (see References)  
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability   
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others 
  Control Strains   Reporter gene Strains  
 Gene GH1: growth hormone 1
 Origin Ikeda developed transgenic rats carrying the human growth hormone (Gh1) driven by murine Wap promoter, originated from Wistar-Imamichi (Ikeda, 1994). After that this transgenic strain has been maintained by artificial insemination to wild-type Wistar-Imamichi. Two lines of this transgenic strain were established named Line 1: characterized by relatively high level of serum Gh1 (high line, NBRPNo.0490), and Line 2: relatively low level of serum Gh1 (low line, NBRPNo.0491). (Oct 30, 2009) 
 Strain Characteristics This strain shows gigantism, polyposia, polyuria and insulin resistance in adipocytes and muscle. (Oct 30, 2009) 
 Breeding Conditions Since this strain’s natural reproductivity is very low, usually it is maintained by artificial insemination, taking sperms from male transgenic rats. (Oct 30, 2009) 
 Genotyping  
 References  Ikeda A, Matsuyama S, Nishihara M, Tojo H, Takahashi M.
Changes in endogenous growth hormone secretion and onset of puberty in transgenic rats expressing human growth hormone gene.
Endocr J. 41(5):523-9, 1994.

Ikeda A, Matsumoto Y, Chang KT, Nakano T, Matsuyama S, Yamanouchi K, Ohta A, Nishihara M, Tojo H, Sasaki F, Takahashi M.
Different female reproductive phenotypes determined by human growth hormone (hGH) levels in hGH-transgenic rats.
Biol Reprod. 56(4):847-51, 1997.

Ikeda A, Chang KT, Matsumoto Y, Furuhata Y, Nishihara M, Sasaki F, Takahashi M.
Obesity and insulin resistance in human growth hormone transgenic rats.
Endocrinology. 139(7):3057-63, 1998.

Furuhata Y, Kagaya R, Hirabayashi K, Ikeda A, Chang KT, Nishihara M, Takahashi M.
Development of obesity in transgenic rats with low circulating growth hormone levels: involvement of leptin resistance.
Eur J Endocrinol. 143(4):535-41, 2000.

Yamanouchi K, Yada E, Hozumi H, Ueno C, Nishihara M.
Analyses of hind leg skeletons in human growth hormone transgenic rats.
Exp Gerontol. 39(8):1179-88, 2004.

Hozumi H, Yamanouchi K, Nishihara M.
Involvement of neuropeptide Y in hyperphagia in human growth hormone transgenic rats.
J Vet Med Sci. 68(9):959-65, 2006.