Japanese
 NBRP Rat No: 0568 Strain Name:  SD-Tg(CAG-HRAS*G12V)250Htsu Commmon Name: SD-Tg(CALNL-HrasGRV)250, Hras250
 Principal Investigator  Hiroyuki Tsuda
 Organization   Nagoya City University Graduate School of Medical Sciences Department of Molecular Toxicology
 Address  1 Kawasumi, Mizuho-cho Mizuho-ku, Nagoya

467-8601 Aichi

  Japan
 Telephone  052-853-8992  Fax:  052-853-8996  htsuda@med.nagoya-cu.ac.jp
 Inbred Generations    
   
 Coat Color
 Deposition Status
 
 albino (c)
  Embryo      Sperm      Live Animals
 Usage Restrictions  The recipient of BIOLOGICAL RESOURCE shall obtain prior written consent concerning usage from the DEPOSITOR. The recipient is requested to cite the literature designated by the DEPOSITOR. 
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability   
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others 
  Control Strains   Reporter gene Strains  
 Gene HRAS: v-Ha-ras Harvey rat sarcoma viral oncogene homolog [ Homo sapiens ]
 Origin This transgenic strain was established by CLEA Japan, Inc. The construct is as follows: CAG promoter, loxP sequence, neomycin resistance gene, loxP sequence and Ha-ras*G12V (HrasV12). It was injected into Jcl:SD embryos (Ueda, 2006). (Oct 30, 2009) 
 Strain Characteristics The transgene is regulated by the Cre/loxP system. Human Ha-ras*G12V oncogene is driven by CAG promoter (Ueda, 2006). (Oct 30, 2009) 
 Breeding Conditions  
 Genotyping  
 References  Nohmi T, Masumura K, Toyoda-Hokaiwado N.
Transgenic rat models for mutagenesis and carcinogenesis.
Genes Environ. 2017 Feb 1;39:11.

Yabushita S, Fukamachi K, Kikuchi F, Ozaki M, Miyata K, Sukata T, Deguchi Y, Tanaka H, Kakehashi A, Kawamura S, Uwagawa S, Wanibuchi H, Suzui M, Alexander DB, Tsuda H.
Twenty-One Proteins Up-Regulated in Human H-ras Oncogene Transgenic Rat Pancreas Cancers are Up-Regulated in Human Pancreas Cancer.
Pancreas. 2013 Aug;42(6):1034-9.

Ueda S, Fukamachi K, Matsuoka Y, Takasuka N, Takeshita F, Naito A, Iigo M, Alexander DB, Moore MA, Saito I, Ochiya T, Tsuda H.
Ductal origin of pancreatic adenocarcinomas induced by conditional activation of a human Ha-ras oncogene in rat pancreas.
Carcinogenesis. 2006 Dec;27(12):2497-510.