Japanese
 NBRP Rat No: 0547 Strain Name: W-Tg(Prl-EGFP)Yamp Commmon Name: Wistar-Tg(Prl-EGFP)Yamp, PRL-EGFP
 Principal Investigator  Jun Arita
 Organization   Graduate School of Medical and Engneering, University of Yamanashi 
 Address  1110, Shimokato, Chuo-city

409-3898 Yamanashi

  Japan
 Telephone  055-273-6730  Fax:  055-273-6730  jarita@yamanashi.ac.jp
 Inbred Generations    
   
 Coat Color
 Deposition Status
 
 albino (c)
  Embryo      Sperm      Live Animals
 Usage Restrictions  In publishing the research results to be obtained by use of the
BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(See references)
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. 
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability   
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others 
  Control Strains   Reporter gene Strains  
 Gene
 Origin A transgenic construct was designed with the rat prolactin promoter (-3221 - 3233) controlling EGFP. Transgenic rats originated from Crlj:WI (Wistar) rats. (Jul 16, 2009) 
 Strain Characteristics In adenohypophysial cells, 55% cells are positive for GFP. Among GFP positive cells, 76% are immunostained with prolactin antibody. (Jul 16, 2009) 
 Breeding Conditions The transgene has no effect on fertility. General breeding and reproduction rate. (Jul 16, 2009) 
 Genotyping 1. PCR analysis on genomic DNA. Primer sequences: Forward: 5'-CTGGCCACTATGTCTTCCTGA-3', Reverse: 5'-TGTAGTTGCCGTCGTCCTTGA-3'. Protocols: 95˚C-10 min, 35 cylcles of (94˚C-1 min, 64˚C-1 min, 72˚C-1 min) Product: about 860-bp. 2. Southern blotting with genomic DNA. EGFP + poly(A) (about 1-kb) is detected with EGFP cDNA probe on BamHI-cut genomic DNA. (Jul 16, 2009) 
 References  Ishida M, Takahashi W, Itoh S, Shimodaira S, Maeda S, Arita J.
Estrogen actions on lactotroph proliferation are independent of a paracrine interaction with other pituitary cell types: a study using lactotroph-enriched cells.
Endocrinology. 148(7):3131-9, 2007.