Japanese
 NBRP Rat No: 0555 Strain NameW-Tg(Slc32a1-YFP*)2Yyan Commmon Name: Wistar-Tg(Slc32a1-YFP*)2Yyan, VGAT-Venus-B
 Principal Investigator  Yuchio Yanagawa
 Organization   Gunma University Graduate School of Medicine Department of Genetic and Behavioral Neuroscience
 Address  3-39-22 Showa-machi, Maebashi

371-8511 Gunma

 Japan
 Telephone  027-220-8040  Fax:  027-220-8046  yuchio@gunma-u.ac.jp
 Inbred Generations   F?+9 (April 2012) 
   
 Coat Color
 Deposition Status
 
 albino (c)
  Embryo      Sperm      Live Animals
 Usage Restrictions  1:The recipient of BIOLOGICAL RESOUCE shall obtain a prior written consent on use of it from the DEPOSITOR.

2: Recipient agrees to cite the following paper in all written publications as related to the Material.

Uematsu M, Hirai Y, Karube F, Ebihara S, Kato M, Abe K, Obata K, Yoshida S, Hirabayashi M, Yanagawa Y & Kawaguchi Y.
Quantitative chemical composition of cortical GABAergic neurons revealed in transgenic Venus-expressing rats.
Cereb Cortex 18, 315-330, 2008.

3: Recipient agrees to describe the following in acknowledgement in all written publications as related to the Materials.

“VGAT-Venus transgenic rats were generated by Drs. Y. Yanagawa, M. Hirabayashi and Y. Kawaguchi in National Institute for Physiological Sciences, Okazaki, Japan, using pCS2-Venus provided by Dr. A. Miyawaki.”

4: Recipient agrees to send the reprints of written publications as related to the Materials to Drs. Yasuo Kawaguchi and Yuchio Yanagawa, when they are published.

(Address)
Yuchio Yanagawa
Department of Genetic and Behavioral Neuroscience
Gunma University Graduate School of Medicine
3-39-22 Showa-machi, Maebashi 371-8511, Japan
E-mail: yuchio@gunma-u.ac.jp

Yasuo Kawaguchi
Division of Cerebral Circuitry
National Institute for Physiological Sciences
Myodaiji, Okazaki 444-8585, Japan
E-mail: yasuo@nips.ac.jp


5: The Venus gene incorporated into VGAT-Venus rats was supplied by Dr. Atsushi Miyawaki at RIKEN, Japan. The following is needed according to the Agreement.

Dr. Atsushi Miyawaki
Laboratory for Cell Function Dynamics
RIKEN Brain Science Institute
2-1 Hirosawa, Wako City
Saitama, 351-0198, JAPAN
Email: matsushi@brain.riken.jp

4-1: RECIPIENT agrees to submit the intended publication to Dr. Atsushi Miyawaki prior to the submission date.
4-2: RECIPIENT agrees to acknowledge Dr. Atsushi Miyawaki in all oral presentations and written publications as related to the MATERIAL. For example, “Venus was developed by Dr. Atsushi Miyawaki at RIKEN, Wako, Japan.”
4-3: RECIPIENT agrees to send the reprints in relation to all oral presentations or written publications as related to the Materials to Dr. Atsushi Miyawaki. 
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability   
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others 
  Control Strains   Reporter gene Strains  
 Gene YFP* (Venus): yellow fluorescent protein variant
 Origin This transgenic rat was established at National Institute for Physiological Sciences in 2004, thereafter introduced to Gunma University in 2006. (Jun 22, 2009) 
 Strain Characteristics This transgenic rat aims to establish Venus-labeled GABAergic neuron. There are 2 strains (A line: NBRP No.0554 and B line: NBRP No.0555) and expression and distribution of YFP* (Venus) are different from between the strains. In the frontal cortex, YFP* was expressed in 98% of GABAergic A line and 93% of that of B line. (Uematsu, 2007) (Jun 22, 2009) 
 Breeding Conditions Identify the transgenic rats by genotyping from progeny of crossing of hemizygous transgenic rat and wild type Wistar rat. (Jun 22, 2009) 
 Genotyping <a href="http://www.anim.med.kyoto-u.ac.jp/nbr/documents/PCR_Gene/Slc32a1-YFP_en.pdf">Genotyping protocol for Slc32a1-YFP*</a>
 
 References  Saito Y, Sugimura T, Yanagawa Y.
Comparisons of Neuronal and Excitatory Network Properties between the Rat Brainstem Nuclei That Participate in Vertical and Horizontal Gaze Holding.
eNeuro 4 September 2017, ENEURO.0180-17.2017

Ueta Y, Otsuka T, Morishima M, Ushimaru M, Kawaguchi Y.
Multiple layer 5 pyramidal cell subtypes relay cortical feedback from secondary to primary motor areas in rats.
Cereb Cortex. 2014 Sep;24(9):2362-76.

Otsuka T, Kawaguchi Y.
Common excitatory synaptic inputs to electrically connected cortical fast-spiking cell networks.
J Neurophysiol. 2013 Aug;110(4):795-806.

Uematsu M, Hirai Y, Karube F, Ebihara S, Kato M, Abe K, Obata K, Yoshida S, Hirabayashi M, Yanagawa Y & Kawaguchi Y.
Quantitative chemical composition of cortical GABAergic neurons revealed in transgenic Venus-expressing rats.
Cereb Cortex 18, 315-330, 2008.