Japanese
 NBRP Rat No: 0604 Strain Name: W-Tg(Tek-GFP)1Soh Commmon Name: Tie2-GFP
 Principal Investigator  Tetsuya Terasaki
 Organization   Tohoku University Graduate School of Pharmaceutical Sciences
 Address  6-3 Aoba Aramaki aza Aoba-ku, Sendai

980-8578 Miyagi

  Japan
 Telephone  022-795-6832  Fax:  022-795-6886  sohtsuki@mail.pharm.tohoku.ac.jp
 Inbred Generations   F2-3 (Jun 2010) 
   
 Coat Color
 Deposition Status
 
 albino (c)
  Embryo      Sperm      Live Animals
 Usage Restrictions  In publishing, a citation of the following literature(s) designated by the DEPOSITOR is requested and an acknowledgment to the DEPOSITOR is also requested.
Ohtsuki S. et al., Vascular endothelium-selective gene induction by Tie2 promoter/enhancer in the brain and retina of a transgenic rat. Pharm Res 2005 Jun;22(6):852-7. 
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability   
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others histology
  Control Strains   Reporter gene Strains  
 Gene Tie2 promoter-GFP (Original plasmid name; pSP14/15.t2hgfpPan5’)
 Origin This transgenic strain was generated by microinjection into fertilized oocytes of Wistar rats (Ohtsuki, 2005). The Tek/GFP plasmid (pSP14/15.t2hgfpPan5’) was a given from Dr. TN Sato of University of Texas Southwestern Medical Center at Dallas (Motoike 2000). The transgene was excised from the plasmid vector by Sal I digestion. (Aug 31, 2010) 
 Strain Characteristics GFP is expressed in capillary endothelial cells under the control of Tek (other name is Tie2) promoter. The blood vessel is visible in this strain. (Aug 31, 2010) 
 Breeding Conditions normal breeding 
 Genotyping Genotyping by PCR analysis.<br />
Extract-N-Amp Tissue PCR kit (SIGMA) Primer 1: 5’-ATTCTCGTGGAACTGGATGG-3’ Primer 2: 5’-GGACAGGTAATGGTTGTCTGG-3’ Signal: 567 bp Cycle Program: 1 cycle of 94C for 3 min, 40 cycles of 94C for 30 s, 55C for 1 min, 72C for 1 min, and a final elongation of 72 C for 2 min. (Aug 31, 2010) 
 References  Motoike T, Loughna S, Perens E, Roman BL, Liao W, Chau TC, Richardson CD, Kawate T, Kuno J, Weinstein BM, Stainier DY, Sato TN.
Universal GFP reporter for the study of vascular development.
Genesis. 2000 Oct;28(2):75-81.

Ohtsuki S, Kamiya N, Hori S, Terasaki T.
Vascular endothelium-selective gene induction by Tie2 promoter/enhancer in the brain and retina of a transgenic rat.
Pharm Res. 2005 Jun;22(6):852-7. Epub 2005 Jun 8.