Japanese
 NBRP Rat No: 0628 Strain Name: F344-Lepm1Kyo Commmon Name: F344 OB rat
 Principal Investigator  Tadao Serikawa
 Organization   Graduate School of Medicine, Kyoto University Institute of Laboratory Animals
 Address  Yoshidakonoe-cho, Sakyo-ku

606-8501 Kyoto

  JAPAN
 Telephone  075-753-4360  Fax:  075-753-4409  serikawa@anim.med.kyoto-u.ac.jp
 Inbred Generations   N10 (April 2012) 
   
 Coat Color
 Deposition Status
 
 albino (c)
  Embryo      Sperm      Live Animals
 Usage Restrictions  The recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
For a commercial use of this resource, a new contract must be concluded between the depositor and the recipient. 
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability   
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others 
  Control Strains   Reporter gene Strains  
 Gene Lep: Leptin
 Origin Established by gene-driven ENU mutagenesis (F344/NSlc). this strain has nonsense mutation （Q92X）in the Lep gene. 
 Strain Characteristics This strain shows obesity.
 
 Breeding Conditions backcrossing to F344/NSlc 
 Genotyping <a href="http://www.anim.med.kyoto-u.ac.jp/nbr/documents/PCR_Gene/lepm1.pdf">Genotyping protocol for Lep</a> 
 References  Aizawa-Abe M, Ebihara K, Ebihara C, Mashimo T, Takizawa A, Tomita T, Kusakabe T, Yamamoto Y, Aotani D, Yamamoto-Kataoka S, Sakai T, Hosoda K, Serikawa T, Nakao K.
Generation of leptin deficient Lepmkyo/Lepmkyo rats and identification of leptin responsive genes in the liver.
Physiol Genomics. 2013 Sep 3;45(17):786-93.

Aizawa-Abe M, Ebihara K, Mashimo T, Kusakabe T, Yamamoto Y, Miyamoto L, Miyawzawa T, Aotani D, Yamamoto-Kataoka S, Sakai T, Ebihara C, Iogawa H, Hosoda K, Serikawa T, Nakao K.
Generation of a genetically obese leptin deficient rat The XVIIIth International Workshop on Genetic Systems in the Rat, Abstract p100, Nov30-Dec3, 2010, Kyoto, Japan.

海老原 健、阿部 恵、真下 知士、海老原 千尋、Valentino Gumbirai, 日下部 徹、細田 公則、芹川 忠夫、中尾 一和
遺伝子改変ラットの解析とトランスレーショナルサイエンス
第47回高血圧関連疾患モデル学会学術総会抄録集, p28, 2011年9月6日, 札幌