English
 NBRP Rat No: 0641 系統名 :    SD-Tg(Neurod6-mCherry,-DTR)Jog 通称: NeuroD6-stop-hDTR
 研究責任者:   Jonathan flint
 所属機関名:   University of Oxford The Wellcome Trust Centre for human Genetics
  住所:   〒 OX3 7BN  

 Oxford  Roosevelt Drive

 
 電話番号:  +44 (0)1865 287772  Fax: +44 (0)1865 287501   jf@well.ox.ac.uk
 近交世代数:  F2 
   
 毛色:  
 保存状況：
 
 
  胚           精子          生体
  利用条件：  利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
 
 分類:   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    その他  
  コマーシャルブリーダー：   
 研究分野:   糖尿病・肥満    脳神経疾患   眼疾患    歯科疾患   循環器系疾患・高血圧
  がん・腫瘍   代謝、内分泌    耳鼻疾患   発生   感染症
   骨形態異常   消化器疾患   皮膚疾患    生殖器疾患   免疫・アレルギー疾患
   行動・学習   血液疾患   泌尿器疾患   薬理学   その他  
  対照動物   レポーター遺伝子動物  
 遺伝子: NeuroD6
 由来: The rats were originally generated by the University of Michigan Transgenic Animal Model Core, University of Michigan. They use a Crl:CD(SD) sub strain of Sprague-Dawley rats, obtained from Charles River Laboratory (Wilmington, MA, USA). The F1 rats have been bred in the UK with Hsd:Sprague Dawley (Harlan laboratories, UK), which are direct descendants of the original 1925 SD-company colony (Madison, Wisconsin, USA).  
 特性など: This rat carries a transgene consisting of the NeuroD6 promoter followed by a 'STOP’ cassette flanked by FRT sites. The stop cassette consists of an mcherry gene encoding for a red fluorescent protein and a kanamycin resistant gene and three SV40polyA adenylation signals. The STOP cassette is followed by a gene encoding for the human diphtheria toxin receptor. This model is yet to be validated.  
繁殖・維持方法: As yet unknown.  
 遺伝診断法: The following primers and protocol can be used to detect the presence of the NeuroD6-stop-hDTR transgene: neo_F AGG ATC TCC TGT CAT CTC ACC TTG CTC CTG neo_R AAG AAC TCG TCA AGA AGG CGA TAG AAG GCG Reagent Volume (ul) Template DNA (10ng/ul) 2 Forward primer (10mMol) 1 Reverse Primer (10mMol) 1 Buffer (10x) 2 dNTP (8mMol) 2 Mg2+ (25mMol) 1.6 Taq Polymerase (Hot Star, Qiagen) 0.1 ddH20 10.3 Total Volume 20 Step Temp (°C) Time DNA activity 1 98 15 minutes Activation of polymerase 2 95 30s Denaturation 3 64 30s Annealing 4 -0.5 °C per cycle 5 72 1 min Extension 6 Go to step 3, 13 times 7 57 30s Annealing 9 72 1 min Extension 10 Got to step 7, 30 times 11 72 10 min final extension 12 4 forever  
  参考文献:  
 追加の系統情報: