| 遺伝診断法: |
MHC遺伝子座およびCblb遺伝子座の遺伝子型の判定は、PCR-RFLPを用いて行う。
MHC遺伝子座（2カ所）： (1)PCR-RFLP primer, D20Rwh1-F:5'-CACCAACGGGACGCAGCGCAT-3', D20Rwh1-R:5'-CAAGCCGCCGCAGGGAGGTG-3', BssHII digestion. (2)PCR-RFLP primer, RT1Db-F:5'-ACGCAGCGCGTGCGGCTTCT-3', RT1Db-R:5'-GCTCCATGAACTCCTTCTGTTTG-3', DdeI digestion.
Cblb遺伝子座： PCR-RFLP primer, Cblb-F:5'-TGC CCC TTC TGT CGC TGT GA-3', Cblb-R:5'-CCT CGG TTT TGA ATC AAC AG-3', TaqI digestion. |
| 参考文献: |
Komeda K, Noda M, Terao K, Kuzuya N, Kanazawa M, Kanazawa Y.
Establishment of two substrains, diabetes-prone and non-diabetic, from Long-Evans Tokushima Lean (LETL) rats.
Endocr J. 1998 Dec;45(6):737-44.
Yokoi N, Komeda K, Wang HY, Yano H, Kitada K, Saitoh Y, Seino Y, Yasuda K, Serikawa T, Seino S.
Cblb is a major susceptibility gene for rat type 1 diabetes mellitus.
Nat Genet. 2002 Aug;31(4):391-4.
Yokoi N, Namae M, Fuse M, Wang HY, Hirata T, Seino S, Komeda K.
Establishment and characterization of the Komeda diabetes-prone rat as a segregating inbred strain.
Exp Anim. 2003 Jul;52(4):295-301 |