Rat Strain Information(FOB: Hand-held Observations) 
Strain Name  ACI-Lystbg-Kyo/Kyo 
Reactivity  2,2,2,2,2,2  
Handling  2,2,2,2,2,2  
Palpebral Closure  1,1,1,1,1,1  
Lacrimation  1,1,1,1,1,1  
Salivation  1,1,1,1,1,1  
Piloerection  1,1,1,1,1,1  
 

Hand-held observations
(Observe animals' reactions when they are removed from their cage and held.)

1) Reactivity: Choose the item from the following list that best describes the ease with which the animal can be removed from its cage (scored).


1: Very easy (animal sits and allows itself to be picked up)
2: Easy/normal (animal does not resist)
3: Somewhat difficult (rat stands)
4: Difficult (animal cringes and becomes rigid or runs around and is difficult to grab)
5: Very difficult (animal attacks)


2) Handling: Choose the item from the following list that best describes the ease with which the animal can be handled after having been removed from cage (scored).


1: Does not resist/ very easy to handle
2: Squeaks or does not squeak but exhibits mild resistance; easy to handle/normal
3: Animal freezes and does not move; becomes rigid in hand
4: Very difficult (animal struggles, squirms, appears distressed, attempts to bite)


3) Palpebral closure: Choose the item from the following list that best describes the closure of the animal's eyes when held in the hand (scored).


1: None/normal/ (eyes are open)
2: Slightly sagging/half shut (eyelids slightly lowered)
3: Shut (eyelids are closed)


4) Lacrimation: Holding the rat, observe areas around eyes and choose the item from the following list that best describes the animal's condition (scored).


1: No lacrimation/normal
2: Moisture only around eyes
3: Moisture flows from eyes


5) Salivation: Holding the rat, observe areas around mouth and choose the item from the following list that best describes the animal's condition (scored).


1: No salivation/normal
2: Moisture only around mouth
3: Moisture flows from mouth


6) Piloerection: Holding the rat, observe coat (for degree of piloerection) and choose the item from the following list that best describes the animal's condition (scored).


1: None/normal
2: Around head and back (mild piloerection only around head and back)
3: Generalized (piloerection all over body)


7) Others (to be recorded as description): Note in detail any findings related to dirtiness of hair cast, bite marks, missing nails, gauntness (stomach can be touched, median vertebrae protrude) or death (findings).
Enter a minus sign (-) in the findings column if no such behavior is observed.