NBRP Rat
Genetic Monitoring
Last Update: March 4, 2010
NBRP-Rat is conducting gene monitoring using SSLP (microsatellite) markers.

Monitored markers
※ Markers are linked to RGD - click for further information.
　
D1Mgh19 D1Rat126 D2Rat202 D2Rat49 D3Rat80 D3Rat24 D4Rat101 D5Mgh23 D5Rat147 D6Rat105
D7Rat103 D9Mit3 D10Rat223 D11Rat63 D14Rat110 D14Rat75 D16Rat35 D18Mit1 D19Rat12 DXRat16

Tested strains and methodology
1. For rats cleaned by embryo transfer: upon transfer to strain maintenance area of our facility
2. For rats cleaned by hysterectomy: upon transfer to the SPF area of our facility
3. For breeding pairs of inbred strains maintained in our facility: alternate year

Sampling of genomic DNA
DNA is requested from the depositor or isolated by NBRP-Rat.
Usually tail samples (about 3mm) for genomic DNA extraction.

Evaluation
Genomic profile is compared to our database and checked for any possible genetic anomaly.


Example: 20 markers and PCR products of standard strains
  ACI/NJcl BN/SsNSlc F344/NSlc IS/Kyo SHR/Izm WKY/Izm LE/Stm
D1Mgh19164 195 326 189 164 164 223
D1Rat126148 123 112 144 112 148 131
D2Rat202195 156 156 189 189 193 154
D2Rat49151 124 157 117 151 121 151
D3Rat80 225 209 225 209 238 209 229
D3Rat24139 139 139 176 176 139 139
D4Rat101228 182 228 184 184 228 198
D5Mgh23121 126 132 117 96 96 128
D5Rat147182 172 174 172 133 180 174
D6Rat105203 225 227 193 195 213 225
D7Rat103218 230 245 239 213 200 243
D9Mit3152 167 173 163 139 139 173
D10Rat223234 244 238 268 246 246 270
D11Rat63250 204 206 225 250 253 250
D14Rat110268 324 283 283 283 283 283
D14Rat75249 259 243 227 220 245 229
D16Rat35272 245 272 274 280 280 262
D18Mit1290 242 247 290 290 249 249
D19Rat12175 214 177 200 173 214 173
DXRat16120 120 126 165 151 165 120