Japanese
NBRP Rat No: 0908
Strain Name
:
LE-Tg(Pdyn-cre)2-9Koba
Commmon Name: Pdyn_Cre RecBAC 2-9ラット
Principal Investigator
Kazuto Kobayashi
Organization
Fukushima Medical University Department of Biomolecular Science, Institute of Biomedical Sciences
Address
1 Hikarigaoka Fukushima-shi
960-1295 Fukushima
Japan
Telephone
024-547-1667
Fax: 024-548-3936
kazuto@fmu.ac.jp
Inbred Generations
F9 (April 2019)
Coat Color
Deposition Status
白黒
Embryo
Sperm
Live Animals
Usage Restrictions
The following specific terms and conditions are requested by the DEPOSITOR. In this event, the RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
Tha RECIPIENT requires a collabration with the DEPOSITOR. Consult with the DEPOSITOR about details beforehand.
Genetic Status
Inbred
Segregating
Congenic
Consomic
Recombinant
Coisogenic
Spont. Mutant
Transgene
Ind. Mutant
Others
Comercial Availability
Research Category
Diabetes Obesity
Neurobiology
Ophthalmology
Dentistry
Cardio- Hypertension
Oncology
Metabolism
Otorhinology
Immunology
Infectious Disease
Osteology
Internal Medicine
Dermatology
Reproduction
Development
Behavior
Hematology
Urology
Pharmacology
Others
Control Strains
Reporter gene Strains
Gene
cre: cre recombinase protein, Pdyn: preprodynorphin
Origin
Background strain: Long-Evans (Iar:Long-Evans, Institute for Animal Reproduction)
This strain was produced by injecting a modified BAC, in which a Cre recombinase was inserted into the third exon of the rat Pdyn gene, into rat zygotes.
Strain Characteristics
This strain is a transgenic rat that expresses Cre recombinase under the control of the Pdyn gene.
Breeding Conditions
Breeding performance is good.
Maintain heterozygous rats by mating them with Long Evans (LE) rats.
Genotyping
The PCR method is used to detect Cre recombinase.
Primer
F: TCGATGCAACGAGTGATGAG, R: TTCGGCTATACGTAACAGGG
PCR conditions
TaKaRa Taq 94℃ 5min→94℃(30cycle) 20sec→56℃ 30sec→72℃ 30sec→4℃ ∞ 482bp
References
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