Japanese
NBRP Rat No: 0979
Strain Name
:
SD-
C5ar1
em22Taoki
Commmon Name:
Principal Investigator
Tomohiro Aoki
Organization
Research Institute, National Cerebral and Cardiovascular Center Department of Molecular Pharmacology
Address
6-1 Kishibeshinmachi, Suita City, Osaka, 564-8565, Japan
564-8565
Japan
Telephone
06-6170-1070
Fax: 06-6170-1416
tomoaoki@ncvc.go.jp
Inbred Generations
8
Coat Color
Deposition Status
白
Embryo
Sperm
Live Animals
Usage Restrictions
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
(Under Review)
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization.
Genetic Status
Inbred
Segregating
Congenic
Consomic
Recombinant
Coisogenic
Spont. Mutant
Transgene
Ind. Mutant
Others
Comercial Availability
Research Category
Diabetes Obesity
Neurobiology
Ophthalmology
Dentistry
Cardio- Hypertension
Oncology
Metabolism
Otorhinology
Immunology
Infectious Disease
Osteology
Internal Medicine
Dermatology
Reproduction
Development
Behavior
Hematology
Urology
Pharmacology
Others
Control Strains
Reporter gene Strains
Gene
C5ar1
Origin
This strain was established by CRISPR/Cas9 system in the Research Institute, National Cerebral and Cardiovascular Center. Genetic background is Slc:SD.
guide RNA
gRNA No1; ATAAGTAACGACAGCAGTGA
gRNA No2; GAGTTTCACTCGGTCCACGA
Strain Characteristics
No abnormalities under normal care.
Breeding Conditions
Maintained by mating between homozygous rats.
Genotyping
Determined by PCR.
Using KOD Plus DNA polymerase (Toyobo).
95°C for 2 min ⇒ (98°C for 10 sec ⇒ 68°C for 15 sec) X 35 cycles ⇒ 68°C for 1 min ⇒ 4°C
Primers 5'-GGTTCTTCAGTATCCCTTGGTAGTGCTGAG-3' and 5'-GGGAGCAGCTAGGCAGTTGTGGC-3'
Band size Wild type 1,198 b.p. Mutant allele 180 b.p.
References
未公表