Japanese
 NBRP Rat No: 0273 Strain Name: W-Tg(CAG-GFP)184Ys Commmon Name: Wistar-Tg(CAG-GFP)184Ys, Wistar-TgN(CAG-GFP)184Ys, Wistar GFP
 Principal Investigator  Eiji Kobayashi
 Organization   Center for Molecular Medicine, Jichi Medical School Division of Organ Replacement Research
 Address  3311-1, Yakushiji, Shimono-shi, Tochigi

329-0498 Tochigi

  Japan
 Telephone  0285-58-7446  Fax:  0285-44-5365  eijikoba@jichi.ac.jp
 Inbred Generations   F21 (March 2012) 
   
 Coat Color
 Deposition Status
 
 albino (c)
  Embryo      Sperm      Live Animals
 Usage Restrictions   
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability   
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others 
  Control Strains   Reporter gene Strains  
 Gene GFP: Green Fluorescent Protein, jellyfish
 Origin This strain was established at YS institute and maintained at PhoenixBio Co., Ltd. and Jichi Medical School. This transgenic rat was originated from Crlj:WI strain. (May 25, 2010) 
 Strain Characteristics This transgenic strain expresses the enhanced green fluorescent protein (EGFP) ubiquitously driven by CAG promoter. 
 Breeding Conditions Good breeding performance. 
 Genotyping Hakamata Y et al., Biochem Biophys Res Commun 2001, 286:779-85<br />
Sense 5'-CCTACGGCAAGCTGACCCTGAAGTT-3'
Anti-sense 5'-AGGACCATGTGATCGCGCTTCTCGT-3'" 
 References  Terada K, Sasaki M, Nagahama H, Kataoka-Sasaki Y, Oka S, Ukai R, Yokoyama T, Iizuka Y, Sakai T, Fukumura S, Tsugawa T, Kocsis JD, Honmou O.
Therapeutic efficacy of intravenous infusion of mesenchymal stem cells in rat perinatal brain injury.
Pediatr Res. 2023 Dec;94(6):1921-1928.

Maeda S, Kawamura T, Sasaki M, Shimamura K, Shibuya T, Harada A, Honmou O, Sawa Y, Miyagawa S.
Intravenous infusion of bone marrow-derived mesenchymal stem cells improves tissue perfusion in a rat hindlimb ischemia model.
Sci Rep. 2022 Oct 10;12(1):16986.

Tabata H, Sasaki M, Kataoka-Sasaki Y, Shinkai N, Ichihara K, Masumori N, Kocsis JD, Honmou O.
Possible role of intravenous administration of mesenchymal stem cells to alleviate interstitial cystitis/bladder pain syndrome in a Toll-like receptor-7 agonist-induced experimental animal model in rat.
BMC Urol. 2021 Nov 13;21(1):156.

Matta A, Karim MZ, Gerami H, Benigno B, Erwin WM.
A comparative study of mesenchymal stem cell transplantation and NTG-101 molecular therapy to treat degenerative disc disease.
Sci Rep. 2021 Jul 20;11(1):14804.

Magota H, Sasaki M, Kataoka-Sasaki Y, Oka S, Ukai R, Kiyose R, Onodera R, Kocsis JD, Honmou O.
Intravenous infusion of mesenchymal stem cells delays disease progression in the SOD1G93A transgenic amyotrophic lateral sclerosis rat model.
Brain Res. 2021 Apr 15:1757:147296.

Yasuda N, Sasaki M, Kataoka-Sasaki Y, Nagahama H, Kocsis JD, Kawaharada N, Honmou O.
Intravenous delivery of mesenchymal stem cells protects both white and gray matter in spinal cord ischemia.
Brain Res. 2020 Nov 15:1747:147040.

Goto T, Miyagawa S, Tamai K, Matsuura R, Kido T, Kuratani T, Shimamura K, Sakaniwa R, Harada A, Sawa Y.
High-mobility group box 1 fragment suppresses adverse post-infarction remodeling by recruiting PDGFRα-positive bone marrow cells.
PLoS One. 2020 Apr 10;15(4):e0230392.

Nakazaki M, Sasaki M, Kataoka-Sasaki Y, Oka S, Suzuki J, Sasaki Y, Nagahama H, Hashi K, Kocsis JD, Honmou O.
Intravenous infusion of mesenchymal stem cells improves impaired cognitive function in a cerebral small vessel disease model.
Neuroscience. 2019 Jun 1:408:361-377.

Sakai T, Sasaki M, Kataoka-Sasaki Y, Oka S, Nakazaki M, Fukumura S, Kobayashi M, Tsutsumi H, Kocsis JD, Honmou O.
Functional recovery after the systemic administration of mesenchymal stem cells in a rat model of neonatal hypoxia-ischemia.
J Neurosurg Pediatr. 2018 Nov 1;22(5):513-522.

Namioka A, Namioka T, Sasaki M, Kataoka-Sasaki Y, Oka S, Nakazaki M, Onodera R, Suzuki J, Sasaki Y, Nagahama H, Kocsis JD, Honmou O.
Intravenous infusion of mesenchymal stem cells for protection against brainstem infarction in a persistent basilar artery occlusion model in the adult rat.
J Neurosurg. 2018 Oct 12;131(4):1308-1316.

Matsuda Y, Sasaki M, Kataoka-Sasaki Y, Takayanagi A, Kobayashi K, Oka S, Nakazaki M, Masumori N, Kocsis JD, Honmou O.
Intravenous Infusion of Bone Marrow-Derived Mesenchymal Stem Cells Reduces Erectile Dysfunction Following Cavernous Nerve Injury in Rats.
Sex Med. 2017 Dec 20. pii: S2050-1161(17)30097-1. [Epub ahead of print]

Nakazaki M, Sasaki M, Kataoka-Sasaki Y, Oka S, Namioka T, Namioka A, Onodera R, Suzuki J, Sasaki Y, Nagahama H, Mikami T, Wanibuchi M, Kocsis JD, Honmou O.
Intravenous infusion of mesenchymal stem cells inhibits intracranial hemorrhage after recombinant tissue plasminogen activator therapy for transient middle cerebral artery occlusion in rats.
J Neurosurg. 2017 Jan 6:1-10.

Sasaki Y, Sasaki M, Kataoka-Sasaki Y, Nakazaki M, Nagahama H, Suzuki J, Tateyama D, Oka S, Namioka T, Namioka A, Onodera R, Mikami T, Wanibuchi M, Kakizawa M, Ishiai S, Kocsis JD, Honmou O.
Synergic Effects of Rehabilitation and Intravenous Infusion of Mesenchymal Stem Cells After Stroke in Rats.
Phys Ther. 2016 Nov;96(11):1791-1798.

Morita T, Sasaki M, Kataoka-Sasaki Y, Nakazaki M, Nagahama H, Oka S, Oshigiri T, Takebayashi T, Yamashita T, Kocsis JD, Honmou O.
Intravenous infusion of mesenchymal stem cells promotes functional recovery in a model of chronic spinal cord injury.
Neuroscience. 2016 Oct 29;335:221-31.

Anna Labedz-Maslowska, Elzbieta Kamycka, Sylwia Bobis-Wozowicz, Zbigniew Madeja, and Ewa K. Zuba-Surma.
Identification of new rat bone marrow- derived population of very small stem cell with Oct-4A and Nanog expression by flow cytometric platforms.
Stem Cells International. 2015. Article ID 531098


Moriyama Y, Takagi N, Itokawa C, Tanonaka K.
Injection of neural progenitor cells attenuates decrease in level of connexin 43 in brain capillaries after cerebral ischemia.
Neurosci Lett. 2013 May 24;543:152-6.

Hern&aacute;ndez Vera R, Vilahur G, Ferrer-Lorente R, Pe&ntilde;a E, Badimon L.
Platelets derived from the bone marrow of diabetic animals show dysregulated endoplasmic reticulum stress proteins that contribute to increased thrombosis.
Arterioscler Thromb Vasc Biol. 2012 Sep;32(9):2141-8.

Y Moriyama, N Takagi and K Tanonaka
Intravenous injection of neural progenitor cells improved depression-like behavior after cerebral ischemia
Translational Psychiatry (2011) 1, e29

Green fluorescent protein-transgenic rat: a tool for organ transplantation research.
Hakamata Y, Tahara K, Uchida H, Sakuma Y, Nakamura M, Kume A, Murakami T, Takahashi M, Takahashi R, Hirabayashi M, Ueda M, Miyoshi I, Kasai N, Kobayashi E.
Biochem Biophys Res Commun. 2001 Aug 31;286(4):779-85.

Intravenous injection of neural progenitor cells improved depression-like behavior after cerebral ischemia
Y Moriyama, N Takagi and K Tanonaka
Translational Psychiatry (2011) 1, e29

Offspring derived from intracytoplasmic injection of transgenic rat sperm.
Hirabayash M, Kato M, Aoto T, Sekimoto A, Ueda M, Miyoshi I, Kasai N, Hochi S.
Transgenic Res. 2002 Apr;11(2):221-8.

Lympho-myeloid chimerism achieved by spleen graft of green fluorescent protein transgenic rat in a combined pancreas transplantation model (TI03-029).
Sakuma Y, Sato Y, Inoue S, Kaneko T, Hakamata Y, Takahashi M, Murakami T, Kobayashi E.
Transpl Immunol. 2004 Jan;12(2):115-22.


Morphologic characterization of green fluorescent protein in embryonic, neonatal, and adult transgenic rats.
Takeuchi K, Sereemaspun A, Inagaki T, Hakamata Y, Kaneko T, Murakami T, Takahashi M, Kobayashi E, Ookawara S.
Anat Rec A Discov Mol Cell Evol Biol. 2003 Oct;274(2):883-6.

Long-lasting donor passenger leukocytes after hepatic and intestinal transplantation in rats.
Inoue S, Tahara K, Kaneko T, Ajiki T, Takeda S, Sato Y, Hakamata Y, Murakami T, Takahashi M, Kaneko M, Kobayashi E.
Transpl Immunol. 2004 Jan;12(2):123-31.