English
 NBRP Rat No: 0273 系統名 :    W-Tg(CAG-GFP)184Ys 通称: Wistar-Tg(CAG-GFP)184Ys, Wistar-TgN(CAG-GFP)184Ys, Wistar GFP
 研究責任者:   小林英司
 所属機関名:  自治医科大学分子病態治療研究センター臓器置換研究部
  住所:   〒329-0498 

 栃木県 栃木県下野市薬師寺3311-1

 日本
 電話番号:  0285-58-7446  Fax: 0285-44-5365  eijikoba@jichi.ac.jp
 近交世代数:  F21 (March 2012) 
   
 毛色:  
 保存状況:
 
 albino (c)
  胚           精子          生体
  利用条件:  なし 
 分類:   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    その他  
  コマーシャルブリーダー:   
 研究分野:   糖尿病・肥満    脳神経疾患   眼疾患    歯科疾患   循環器系疾患・高血圧
  がん・腫瘍   代謝、内分泌    耳鼻疾患   発生   感染症
   骨形態異常   消化器疾患   皮膚疾患    生殖器疾患   免疫・アレルギー疾患
   行動・学習   血液疾患   泌尿器疾患   薬理学   その他  
  対照動物   レポーター遺伝子動物  
 遺伝子: GFP: Green Fluorescent Protein, jellyfish
 由来: (株)ワイエス研究所で作製、(株)フェニックスバイオ(旧ワイエス研究所)および自治医科大学で維持している。 Crlj:WI (日本チャールス・リバーのWistar)由来のトランスジェニックラット。 
 特性など: CAGベクターのプロモーター制御下で、全身の組織細胞にGFP(緑色蛍光たんぱく質)を発現する。
遺伝子組換え生物
遺伝子組換え生物等の第二種使用等、大臣確認:不要、宿主:ラット、供与核酸:GFP遺伝子、施行規則第十六条第一号、第二号又は第四号に基づく使用等:該当なし、拡散防止区分:P1A、組換えの分類:TG 
繁殖・維持方法: 繁殖成績良好 
 遺伝診断法: Hakamata Y et al., Biochem Biophys Res Commun 2001, 286:779-85参照
Sense 5'-CCTACGGCAAGCTGACCCTGAAGTT-3' Anti-sense 5'-AGGACCATGTGATCGCGCTTCTCGT-3'" 
  参考文献: Matsuda Y, Sasaki M, Kataoka-Sasaki Y, Takayanagi A, Kobayashi K, Oka S, Nakazaki M, Masumori N, Kocsis JD, Honmou O.
Intravenous Infusion of Bone Marrow-Derived Mesenchymal Stem Cells Reduces Erectile Dysfunction Following Cavernous Nerve Injury in Rats.
Sex Med. 2017 Dec 20. pii: S2050-1161(17)30097-1. [Epub ahead of print]

Nakazaki M, Sasaki M, Kataoka-Sasaki Y, Oka S, Namioka T, Namioka A, Onodera R, Suzuki J, Sasaki Y, Nagahama H, Mikami T, Wanibuchi M, Kocsis JD, Honmou O.
Intravenous infusion of mesenchymal stem cells inhibits intracranial hemorrhage after recombinant tissue plasminogen activator therapy for transient middle cerebral artery occlusion in rats.
J Neurosurg. 2017 Jan 6:1-10.

Sasaki Y, Sasaki M, Kataoka-Sasaki Y, Nakazaki M, Nagahama H, Suzuki J, Tateyama D, Oka S, Namioka T, Namioka A, Onodera R, Mikami T, Wanibuchi M, Kakizawa M, Ishiai S, Kocsis JD, Honmou O.
Synergic Effects of Rehabilitation and Intravenous Infusion of Mesenchymal Stem Cells After Stroke in Rats.
Phys Ther. 2016 Nov;96(11):1791-1798.

Morita T, Sasaki M, Kataoka-Sasaki Y, Nakazaki M, Nagahama H, Oka S, Oshigiri T, Takebayashi T, Yamashita T, Kocsis JD, Honmou O.
Intravenous infusion of mesenchymal stem cells promotes functional recovery in a model of chronic spinal cord injury.
Neuroscience. 2016 Oct 29;335:221-31.

Anna Labedz-Maslowska, Elzbieta Kamycka, Sylwia Bobis-Wozowicz, Zbigniew Madeja, and Ewa K. Zuba-Surma.
Identification of new rat bone marrow- derived population of very small stem cell with Oct-4A and Nanog expression by flow cytometric platforms.
Stem Cells International. 2015. Article ID 531098


Moriyama Y, Takagi N, Itokawa C, Tanonaka K.
Injection of neural progenitor cells attenuates decrease in level of connexin 43 in brain capillaries after cerebral ischemia.
Neurosci Lett. 2013 May 24;543:152-6.

Hernández Vera R, Vilahur G, Ferrer-Lorente R, Peña E, Badimon L.
Platelets derived from the bone marrow of diabetic animals show dysregulated endoplasmic reticulum stress proteins that contribute to increased thrombosis.
Arterioscler Thromb Vasc Biol. 2012 Sep;32(9):2141-8.

Y Moriyama, N Takagi and K Tanonaka
Intravenous injection of neural progenitor cells improved depression-like behavior after cerebral ischemia
Translational Psychiatry (2011) 1, e29

Green fluorescent protein-transgenic rat: a tool for organ transplantation research.
Hakamata Y, Tahara K, Uchida H, Sakuma Y, Nakamura M, Kume A, Murakami T, Takahashi M, Takahashi R, Hirabayashi M, Ueda M, Miyoshi I, Kasai N, Kobayashi E.
Biochem Biophys Res Commun. 2001 Aug 31;286(4):779-85.

Intravenous injection of neural progenitor cells improved depression-like behavior after cerebral ischemia
Y Moriyama, N Takagi and K Tanonaka
Translational Psychiatry (2011) 1, e29

Offspring derived from intracytoplasmic injection of transgenic rat sperm.
Hirabayash M, Kato M, Aoto T, Sekimoto A, Ueda M, Miyoshi I, Kasai N, Hochi S.
Transgenic Res. 2002 Apr;11(2):221-8.

Lympho-myeloid chimerism achieved by spleen graft of green fluorescent protein transgenic rat in a combined pancreas transplantation model (TI03-029).
Sakuma Y, Sato Y, Inoue S, Kaneko T, Hakamata Y, Takahashi M, Murakami T, Kobayashi E.
Transpl Immunol. 2004 Jan;12(2):115-22.


Morphologic characterization of green fluorescent protein in embryonic, neonatal, and adult transgenic rats.
Takeuchi K, Sereemaspun A, Inagaki T, Hakamata Y, Kaneko T, Murakami T, Takahashi M, Kobayashi E, Ookawara S.
Anat Rec A Discov Mol Cell Evol Biol. 2003 Oct;274(2):883-6.

Long-lasting donor passenger leukocytes after hepatic and intestinal transplantation in rats.
Inoue S, Tahara K, Kaneko T, Ajiki T, Takeda S, Sato Y, Hakamata Y, Murakami T, Takahashi M, Kaneko M, Kobayashi E.
Transpl Immunol. 2004 Jan;12(2):123-31. 
 追加の系統情報:  Wistar