NBRP Rat No: 0003 Strain NameDMY/Kyo Commmon Name: DMY, demyelination rat
 Principal Investigator  Tadao Serikawa
 Organization   Graduate School of Medicine, Kyoto University Institute of Laboratory Animals
 Address  Yoshidakonoe-cho, Sakyo-ku

606-8501 Kyoto

 Telephone  075-753-4360  Fax:  075-753-4409  serikawa@anim.med.kyoto-u.ac.jp
 Inbred Generations   F?+46 (March 2012) 
 Coat Color
 Deposition Status
 albino (c)
  Embryo      Sperm      Live Animals
 Usage Restrictions  The recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
For a commercial use of this resource, a new contract must be concluded between the depositor and the recipient. 
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability   
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others 
  Control Strains   Reporter gene Strains  
 Origin From a closed colony of Sprague Dawley (SD) rats in the laboratory animal facilities of the Universitat Autónoma de Barcelona (Bellaterra Campus) in 1991. Via Institute Pasteur, Paris, to Kyoto University (1996). 
 Strain Characteristics DMY rats exhibit hind limb ataxia and severe myelin breakdown in the CNS during the late stage of postnatal myelination (Kuramoto, 1996; Kuwamura, 2004). By genetic analysis, the causative gene <i>dmy</i> (demyelination) was mapped to rat chromosome 17, very close to the prolactin (<i>Prl</i>) locus (Kuramoto, 1996) and the corresponding mouse genomic region was determined on mouse chromosome 13 (Kitada, 2000). DMY rats show an abnormal ion accumulation and significant upregulation of iron regulatory proteins in the spinal cord white matter (Izawa, 2010). (Oct 8, 2010) 
 Breeding Conditions Maintained in heterozygous condition. 
 Genotyping <a href="http://www.anim.med.kyoto-u.ac.jp/nbr/documents/PCR_Gene/Mrs2_dmy_en.pdf">Genotyping protocol for Mrs2</a> 
 References  Kuramoto T, Sotelo C, Yokoi N, Serikawa T, Gonalons Sintes E, Canto Martorell J, Guenet J-L.
A rat mutation producing demyelination (dmy) maps to chromosome 17.
Mamm Genome. 7: 890-894, 1996.

Kitada K. et al
Determination of the mouse homologous region for the rat dmy locus.
J Exp Anim. Sci. 41:40-43, 2000.

Kuwamura M, Kanehara T, Tokuda S, Kumagai D, Yamate J, Kotani T, Nakane Y, Kuramoto T, Serikawa T
Immunohistochemical and morphometrical studies on myelin breakdown in the demyelination (dmy) mutant rat.
Brain Res. 2004 Oct 1;1022(1-2):110-6.

Izawa T, Yamate J, Franklin RJ, Kuwamura M.
Abnormal iron accumulation is involved in the pathogenesis of the demyelinating dmy rat but not in the hypomyelinating mv rat.
Brain Res. 2010 Aug 19;1349:105-14.

Kuramoto T, Kuwamura M, Tokuda S, Izawa T, Nakane Y, Kitada K, Akao M, Gu&eacute;net JL, Serikawa T.
A mutation in the gene encoding mitochondrial Mg&sup2;+ channel MRS2 results in demyelination in the rat.
PLoS Genet. 2011 Jan 6;7(1):e1001262.