Strain Name	Body Position	Respiration	Tonic Involuntary Movement Cage	Vocalization	Palpebral Closure Cage
ACI/NHok	S,S,S,S,S,S	1,1,1,1,1,1	1,1,1,1,1,1	1,1,1,1,1,1	1,1,1,1,1,1
ACI/NJcl	S,A,A,A,A,A	1,1,1,1,1,1	1,1,1,1,1,1	1,1,1,1,1,1	1,3,3,3,3,3
ACI/NKyo	A,A,A,A,A,A	1,1,1,1,1,1	1,1,1,1,1,1	1,1,1,1,1,1	2,2,2,2,2,2
ACI/Nkyo(F)	R,S,S,S,S,S	1,1,1,1,1,1	1,1,1,1,1,1	1,1,1,1,1,1	1,1,1,1,1,1
ACI/NMna	S,S,S,S,S,S	1,1,1,1,1,1	1,1,1,1,1,1	1,1,1,1,1,1	1,1,1,1,1,1
ACI/NSlc	R,R,R,R,R,R	1,1,1,1,1,1	1,1,1,1,1,1	1,1,1,1,1,1	1,1,1,1,1,1
ACI-Lystbg-Kyo/Kyo	S,S,S,S,S,S	1,1,1,1,1,1	1,1,1,1,1,1	1,1,1,1,1,1	2,2,2,2,2,2
ALB/Hok(F)	R,A,R,S,S,R	1,1,1,1,1,1	1,1,1,1,1,1	1,1,1,1,1,1	1,3,1,1,1,1
BN/1Hok	S,S,S,S,S,S	1,1,1,1,1,1	1,1,1,1,1,1	1,1,1,1,1,1	1,1,1,1,1,1
BN/fMaiHok	S,S,S,A,A,A	1,1,1,1,1,1	1,1,1,1,1,1	1,1,1,1,1,1	1,1,1,2,3,2
BN/KtsSlc	S,S,S,S,S,S	1,1,1,1,1,1	1,1,1,1,1,1	1,1,1,1,1,1	1,1,1,1,1,1
BN/KunKtsSlc	S,S,A,S,A,S	1,1,1,1,1,1	1,1,1,1,1,1	1,1,1,1,1,1	2,2,3,3,3,2
BN/Seac	A,A,A,A,S,A	1,1,1,1,1,1	1,1,1,1,1,1	1,1,1,1,1,1	3,3,2,3,2,2,
BN/SsNHsd	S,A,A,S,A,A	1,1,1,1,1,1	1,1,1,1,1,1	1,1,1,1,1,1	1,3,3,1,2,3
BN/SsNSlc	S,S,S,R,R,R	1,1,1,1,1,1	1,1,1,1,1,1	1,1,1,1,1,1	1,1,1,1,1,1
BUF/Mna	S,S,S,S,S,S	1,1,1,1,1,1	1,1,1,1,1,1	1,1,1,1,1,1	1,1,1,1,1,1
DA/Slc	S,S,S,S,S,S	1,1,1,1,1,1	1,1,1,1,1,1	1,1,1,1,1,1	1,1,1,1,1,1
Dahl S/JrSeac	A,A,S,S,R,S	1,1,1,1,1,1	1,1,1,1,1,1	1,1,1,1,1,1	33,1,1,1,1
DDI/Ddia	S,S,S,S,S,S	1,1,1,1,1,1	1,1,1,1,1,1	1,1,1,1,1,1	1,1,1,1,1,1
DMY/Kyo	A,A,A,A,A,A	1,1,1,1,1,1	1,1,1,1,1,1	1,1,1,1,1,1	3,3,3,3,3,3
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Home-cage measurements
(Observe the animals from outside the cages, taking care not to agitate them. Do not touch the animals.)

1) Body position: Choose the word from the following list that best describes the animal's posture (classification).

S (sitting or standing): Sitting or normally standing
A (asleep): lying on side curled up or crouching
R (rearing): Standing on hindlimbs
H (hunchback): Back is rounded, even when walking, and animal appears to be holding stomach
L (lying): Lying on side, limbs in air
F (flattened): Animal is spread out with abdomen pressed to floor
C (catalepsy): Animal is in a cataleptic-like state; may be 1 of a variety of postures, but animal must maintain an unnatural posture
Note: Only an assessment of S, A or, if of a mild degree, R is considered normal.

2) Respiration: Choose the item from the following list that best describes the rate and extent of breathing (score).

1: Normal
2: Slightly incomplete/fast and shallow, bradypnea (breathing either fast and shallow or slow)
3: Moderately incomplete/rapid breathing, difficulty breathing (breathing very fast and shallow or very shallow and labored in appearance)
4: Severely incomplete/wheezing, breathing with mouth open (wheezing or breathing with mouth open)
5: Weak breathing (breathing very little)
3) Clonic involuntary movement: Choose the applicable item from the following list (score).
1: None/normal
2: Repetitive mouth/jaw motion
3: Twitching of limb, auricles or head
4: Generalized tremors
5: Clonic seizure
6: Trembling

4) Tonic involuntary movement: Choose the applicable item from the following list (scored).

1: None/normal
2: Extending contraction (limb or limbs extends and becomes rigid)
3: Sudden jumping (all limbs come off floor)
4: Tonic extending convulsion
5: Tonic convulsions seen with respiratory distress, seizure or death

5) Vocalization: Check for spontaneous vocalization. Choose the applicable item from the following list (scored).

1: No vocalization/normal
2: Vocalization noted

6) Palpebral closure: Choose the item from the following list that best describes the closure of the animal's eyes within the cage (scored).

1: Open
2: Slightly sagging/half shut (eyelids slightly lowered)
3: Shut (eyelids are closed)