Japanese
 NBRP Rat No: 0642 Strain Name: SD-Tg(Camk2a-FLPo)Jog Commmon Name: CamK2a-flpO
 Principal Investigator  Jonathan flint
 Organization    University of Oxford  The Wellcome Trust Centre for human Genetics
 Address  OX3 7BN Roosevelt Drive

 Oxford

  UK
 Telephone  44 (0)1865 287772  Fax:  44 (0)1865 287501  jf@well.ox.ac.uk
 Inbred Generations   More than 10 
   
 Coat Color
 Deposition Status
 
 white
  Embryo      Sperm      Live Animals
 Usage Restrictions  In publishing, an acknowledgment to the DEPOSITOR is requested. 
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability   
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others 
  Control Strains   Reporter gene Strains  
 Gene CAMK2A
 Origin The rats were originally generated by the University of Michigan Transgenic Animal Model Core, University of Michigan. They use a Crl:CD(SD) sub strain of Sprague-Dawley rats, obtained from Charles River Laboratory (Wilmington, MA, USA). The F1 rats have been bred in the UK with Hsd:Sprague Dawley (Harlan laboratories, UK), which are direct descendants of the original 1925 SD-company colony (Madison, Wisconsin, USA).
The detailed FLPo expression pattern has not yet been analyzed. (Jun, 2017)  
 Strain Characteristics This transgene consists of approximately 6kb of the CamK2a promoter followed by a gene encoding FLP, optimised for mamalian exprssion (FLPo). This rat remains unvalidated. The detailed expression pattern of FLPo has not yet been examined. 
 Breeding Conditions Both males and females are fertile and generate good litters sizes of 10-15 pups at 50% heterozygosity when bred with WT mates.  
 Genotyping The CamK2a-FLPo transgene can be detected usingthe follwoign primers and PCR protocol:
CamK_flp_F aga atg tgg cac cca cta gc
CamK_flp_R tct tct tgc tgt ggc tgt tg

Reagent Volume (ul)
Template DNA (10ng/ul) 2
Forward primer (10mMol) 1
Reverse Primer (10mMol) 1
Buffer (10x) 2
dNTP (8mMol) 2
Mg2+ (25mMol) 1.6
Taq Polymerase (Hot Star, Qiagen) 0.1
ddH20 10.3
Total Volume 20


Step Temp (°C) Time DNA activity
1 98 15 minutes Activation of polymerase
2 95 30s Denaturation
3 64 30s Annealing
4 -0.5 °C per cycle
5 72 1 min Extension
6 Go to step 3, 13 times
7 57 30s Annealing
9 72 1 min Extension
10 Got to step 7, 30 times
11 72 10 min final extension
12 4 forever
 
 References