English
 NBRP Rat No: 0642 系統名 :    SD-Tg(Camk2a-FLPo)Jog 通称: CamK2a-flpO
 研究責任者:   Jonathan flint
 所属機関名:   University of OxfordThe Wellcome Trust Centre for human Genetics
  住所:   〒 

 Oxford OX3 7BN Roosevelt Drive

 
 電話番号:  44 (0)1865 287772  Fax: 44 (0)1865 287501  jf@well.ox.ac.uk
 近交世代数:  More than 10 
   
 毛色:  
 保存状況:
 
 white
  胚           精子          生体
  利用条件:  利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
 
 分類:   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    その他  
  コマーシャルブリーダー:   
 研究分野:   糖尿病・肥満    脳神経疾患   眼疾患    歯科疾患   循環器系疾患・高血圧
  がん・腫瘍   代謝、内分泌    耳鼻疾患   発生   感染症
   骨形態異常   消化器疾患   皮膚疾患    生殖器疾患   免疫・アレルギー疾患
   行動・学習   血液疾患   泌尿器疾患   薬理学   その他  
  対照動物   レポーター遺伝子動物  
 遺伝子: CAMK2A
 由来: The rats were originally generated by the University of Michigan Transgenic Animal Model Core, University of Michigan. They use a Crl:CD(SD) sub strain of Sprague-Dawley rats, obtained from Charles River Laboratory (Wilmington, MA, USA). The F1 rats have been bred in the UK with Hsd:Sprague Dawley (Harlan laboratories, UK), which are direct descendants of the original 1925 SD-company colony (Madison, Wisconsin, USA). ※なお、FLPo発現パターンの詳細な解析はされていないとのこと(2017年6月) 
 特性など: This transgene consists of approximately 6kb of the CamK2a promoter followed by a gene encoding FLP, optimised for mamalian exprssion (FLPo). This rat remains unvalidated. The detailed expression pattern of FLPo has not yet been examined. 
繁殖・維持方法: Both males and females are fertile and generate good litters sizes of 10-15 pups at 50% heterozygosity when bred with WT mates.  
 遺伝診断法: The CamK2a-FLPo transgene can be detected usingthe follwoign primers and PCR protocol: CamK_flp_F aga atg tgg cac cca cta gc CamK_flp_R tct tct tgc tgt ggc tgt tg Reagent Volume (ul) Template DNA (10ng/ul) 2 Forward primer (10mMol) 1 Reverse Primer (10mMol) 1 Buffer (10x) 2 dNTP (8mMol) 2 Mg2+ (25mMol) 1.6 Taq Polymerase (Hot Star, Qiagen) 0.1 ddH20 10.3 Total Volume 20 Step Temp (°C) Time DNA activity 1 98 15 minutes Activation of polymerase 2 95 30s Denaturation 3 64 30s Annealing 4 -0.5 °C per cycle 5 72 1 min Extension 6 Go to step 3, 13 times 7 57 30s Annealing 9 72 1 min Extension 10 Got to step 7, 30 times 11 72 10 min final extension 12 4 forever  
  参考文献:  
 追加の系統情報: