Japanese
 NBRP Rat No: 0698 Strain Name: DA.PVG.1AV1-(OT24.18-D10Rat93)/Kini Commmon Name: DA.PVG.1AV1-Eae18b
 Principal Investigator  Tomas Olsson
 Organization   Karolinska Institutet 
 Address  CMM, Karolinska University Hospital, 171 76,

L8:04, Stockholm

  Sweden
 Telephone  +46 8 51776258  Fax:  +46 8 51775562  tomas.olsson@ki.se
 Inbred Generations   Congenic strain was establiesh from the 10th generation advanced intercross line after 8 generations of backcrossing in 2008. Since then, the strain was kept as inbred.  
   
 Coat Color
 Deposition Status
 
 Agouti
  Embryo      Sperm      Live Animals
 Usage Restrictions  In publishing, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Development and the characterization of the strain has been published in this article:
Adzemovic MZ, Ockinger J, Zeitelhofer M, Hochmeister S, Beyeen AD, Paulson A, Gillett A, Hedreul MT, Covacu R, Lassmann H, Olsson T, Jagodic M. Expression of Ccl11 Associates with Immune Response Modulation and Protection against Neuroinflammation in Rats. PLoS One. 2012;7(7):e39794. Epub 2012 Jul 16. PMID: 22815714. 
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability   
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others 
  Control Strains   Reporter gene Strains  
 Gene > 13.5 Mb
 Origin Inbred Dark Agouti (DA) rats were originally obtained from the Zentralinstitut für Versuchstierzucht (Hannover, Germany) and Piebald Virol Glaxo(PVG) rats from Harlan UK Limited (Blackthorn, UK). Animals for congenic breeding were selected from the 10th generation of an advanced intercross line (AIL) originating from the EAE-susceptible DA and EAE-resistant PVG.av1 rat strains. Selected males containing a PVG.av1 fragment in the Eae18b region (from OT24.18 to D10Rat93 marker, at 68.36 Mb and 81.9 Mb, respectively) were backcrossed with DA females for 8 generations, and offspring was genotyped using microsatellite markers in each generation. In the 8th generation, heterozygous males and females were crossed to obtain the experimental population of homozygous congenic rats and littermate controls. 
 Strain Characteristics Protection against experimental autoimmune encephalomyelitis (EAE) compared to the susceptible parental DA strain and displays higher CCL11 expression. 
 Breeding Conditions Good breeding performance.  
 Genotyping Flanking markers inside the congenic fragment: OT24.18-D10Rat93. 
 References  Jagodic M, Becanovic K, Sheng JR, Wu X, Bäckdahl L, Lorentzen JC, Wallström E, Olsson T. An advanced intercross line resolves Eae18 into two narrow quantitative trait loci syntenic to multiple sclerosis candidate loci. J Immunol. 2004 Jul 15;173(2):1366-73. PMID: 15240732.
Ockinger J, Stridh P, Beyeen AD, Lundmark F, Seddighzadeh M, Oturai A, Sørensen PS, Lorentzen AR, Celius EG, Leppä V, Koivisto K, Tienari PJ, Alfredsson L, Padyukov L, Hillert J, Kockum I, Jagodic M, Olsson T. Genetic variants of CC chemokine genes in experimental autoimmune encephalomyelitis, multiple sclerosis and rheumatoid arthritis. Genes Immun. 2010 Mar;11(2):142-54. Epub 2009 Oct 29. PMID: 19865101.
Adzemovic MZ, Ockinger J, Zeitelhofer M, Hochmeister S, Beyeen AD, Paulson A, Gillett A, Hedreul MT, Covacu R, Lassmann H, Olsson T, Jagodic M. Expression of Ccl11 Associates with Immune Response Modulation and Protection against Neuroinflammation in Rats. PLoS One. 2012;7(7):e39794. Epub 2012 Jul 16. PMID: 22815714.