Japanese
 NBRP Rat No: 0093 Strain Name: DOP/Nem Commmon Name: DOP, dilute-opisthotonus
 Principal Investigator  Yoshiko Takagishi
 Organization   Research Institute of Environmental Medicine, Nagoya University Department of Teratology and Genetics
 Address  Furo-cho, Chigusa-ku, Nagoya

464－8601 Aichi

  JAPAN
 Telephone  052-789-3874  Fax:  052-789-3876  taka@riem.nagoya-u.ac.jp
 Inbred Generations   F25+?+16 (May 2009) 
   
 Coat Color
 Deposition Status
 
 gray
  Embryo      Sperm      Live Animals
 Usage Restrictions  In publishing, a citation of the following literature(s) designated by the DEPOSITOR is requested (*). 
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability   
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others 
  Control Strains   Reporter gene Strains  
 Gene Myo5a: myosin Va
 Origin Founded from a breeding colony of Wistar by Ohno at Yagi Memorial Park in 1988. A congenic line BN-dop and an inbred line DOP-dop were established.  
 Strain Characteristics Normal behaviour until day 10, thereafter, excessive spinning around on smooth surfaces and tendency to be off balance. Slight convulsive limb movements. Can't regain upright posture. Limb convulsion associated with opisthotonus becomes tetanic. Hyperacuety. Dead after 3 weeks. Lighter coat color pigmentation. Controlled by an autosomal recessive gene (Dekker-Ohno et al. 1993).  
 Breeding Conditions Maintained by crossing heterozygotes of the dop locus (segregating inbred strain). 
 Genotyping <a href="http://www.anim.med.kyoto-u.ac.jp/nbr/documents/PCR_Gene/Myo5_dop_en.pdf">Genotyping protocol for Myo5</a> 
 References  * Dekker-Ohno K, Oda S, Yamamura H, Kondo K.
An ataxic mutant rat with dilute coat color.
Lab Anim Sci. 1993 Aug;43(4):370-2

* Futaki S, Takagishi Y, Hayashi Y, Ohmori S, Kanou Y, Inouye M, Oda S, Seo H, Iwaikawa Y, Murata Y.
Identification of a novel myosin-Va mutation in an ataxic mutant rat, dilute-opisthotonus.
Mamm Genome. 2000 Aug;11(8):649-55.

* Dekker-Ohno K, Hayasaka S, Takagishi Y, Oda S, Wakasugi N, Mikoshiba K, Inouye M, Yamamura H.
Endoplasmic reticulum is missing in dendritic spines of Purkinje cells of the ataxic mutant rat.
Brain Res. 1996 Apr 1;714(1-2):226-30

* Miyata M, Finch EA, Khiroug L, Hashimoto K, Hayasaka S, Oda SI, Inouye M, Takagishi Y, Augustine GJ, Kano M.
Local calcium release in dendritic spines required for long-term synaptic depression.
Neuron. 2000 Oct;28(1):233-44