NBRP Rat No: 0028 Strain NameKZC/Tky Commmon Name: KZC, Komeda Zucker Creeping rat
 Principal Investigator  Eiichi Kawamoto
 Organization   Tokyo Medical University Institution: Animal Research Center
 Address  6-1-1 Shinjuku, Shinjuku-ku,

160-8402 Tokyo

 Telephone  03-3351-6141  Fax:  03-3350-8689  kawamoto@tokyo-med.ac.jp
 Inbred Generations   F45 
 Coat Color
 Deposition Status
 black hooded (a,B,C,h)
  Embryo      Sperm      Live Animals
 Usage Restrictions   
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability   
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others 
  Control Strains   Reporter gene Strains  
 Gene Reln: reelin
 Origin Komeda Zucker Creeping rat derived from a closed colony of the Zucker fatty rat by spontaniously mutation at Tokyo Medical College in 1983. 
 Strain Characteristics The animals exhibiting severe ataxia with staggering gait and tremor at about 14 days of age, and die around 4 weeks of age. The cerebellum of the mutants shows severe hypoplasia with the reduction of the molecular and granular layers and malposition of Purkinje cells in the white matter. The abnormal pathological changes are also detected in the cerebrum, including the absence of a well-defined outer molecular layer, no typical lamination, and irregular cell arrangement in the hippocampus.  
 Breeding Conditions Homozygous <i>Reln</i rats are difficult to breed. Maintained in heterozygous condition. 
 Genotyping One base insertion detected by PCR-direct sequencing. 
 References  Nakanishi S, Kuramoto T, Kashiwazaki N, Yokoi N.
Genetic profiling of two phenotypically distinct outbred rats derived from a colony of the Zucker fatty rats maintained at the Tokyo Medical University.
Exp Anim. 2016 Oct 31. [Epub ahead of print]

Yokoi N, Namae M, Wang HY, Kojima K, Fuse M, Yasuda K, Serikawa T, Seino S, Komeda K.
Rat neurological disease creeping is caused by a mutation in the reelin gene.
Brain Res Mol Brain Res. 10;112(1-2):1-7, 2003.

Yokoi N, Shimizu S, Ishibashi K, Kitada K, Iwama H, Namae M, Sugawara M, Serikawa T, Komeda K.
Genetic mapping of the rat mutation creeping and evaluation of its positional candidate gene reelin.
Mamm Genome. 11(2):111-4, 2000.

Ishibashi K, Komeda K, Sekiguchi F, Kanazawa Y.
Creeping: a new mutant rat with neurological disease.
Lab Anim Sci. 39(2):132-6, 1989.