Japanese
 NBRP Rat No: 0196 Strain NameKDP-Tg(H2Kd-Cblb)1Nyo Commmon Name: 
 Principal Investigator  Susumu Seino
 Organization   Kobe University Graduate School of Medicine 
 Address  7-5-1 Kusunoki-cho, Chuou-Ku, Kobe

650-0017 Hyogo

 Japan
 Telephone  078-382-5364  Fax:  078-382-5379  seino@med.kobe-u.ac.jp, yokoi@med.kobe-u.ac.jp
 Inbred Generations   N5 
   
 Coat Color
 Deposition Status
 
 black hooded (a,B,C,h)
  Embryo      Sperm      Live Animals
 Usage Restrictions  The recipients shall cite the references of the resource and acknowledge the developer as the source of the resource in the publications. Depending on the project of the recipients, a collaborative arrangement with the developer could be required. In this case, the recipients shall discuss with the developer. The recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. 
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability   
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others 
  Control Strains   Reporter gene Strains  
 Gene Cblb: Casitas B-lineage lymphoma b
 Origin The transgenic rats were established by injection of transgene consisting of wild-type <i>Cblb</i> into Sprague Dawley (Slc:SD) rat fertile eggs in 2000, followed by backcrossing the transgenic rats onto KDP rats. This transgenic rat expresses the wild-type cDNA of <i>Cblb</i> gene under the control of mouse H2-Kd promoter. (Jul 2009) 
 Strain Characteristics KDP is a spontaneous animal model of human type 1 diabetes and a nonsense mutation in <i>Cblb</i> was identified as a causative gene. To confirm that the <i>Cblb</i> mutation is responsible for the phenotype of the KDP rat, wild-type <i>Cblb</i> gene was introduced to KDP rats as rescue experiments. As a result, this transgenic strain significantly suppressed development of the KDP phenotype. (Yokoi, 2002) (Jul 2009) 
 Breeding Conditions Homozygous <i>Cblb</i> rats are usually infertile in both sexes and are therefore maintained by crossing hemizygous individuals (segregating inbred strain). 
 Genotyping  
 References  Yokoi N, Komeda K, Wang HY, Yano H, Kitada K, Saitoh Y, Seino Y, Yasuda K, Serikawa T, Seino S.
Cblb is a major susceptibility gene for rat type 1 diabetes mellitus.
Nat Genet. 31(4):391-4, 2002

Yokoi N, Namae M, Fuse M, Wang HY, Hirata T, Seino S, Komeda K.
Establishment and characterization of the Komeda diabetes-prone rat as a segregating inbred strain.
Exp Anim. 52(4):295-301, 2003