Japanese
 NBRP Rat No: 0603 Strain Name: W-Tg(Gh1as)Nibs Commmon Name: ミニラット　Nts:Mini rat
 Principal Investigator  Tetsuo Nunoya
 Organization   Nippon Institute for Biological Science  
 Address  9-2221-1, Shinmachi, Ome

198-0024 Tokyo

  Japan
 Telephone  0428-33-1056  Fax:  0428-33-1036  s-nobuko@nibs.or.jp
 Inbred Generations   F46 (May 2010) 
   
 Coat Color
 Deposition Status
 
 albino (c)
  Embryo      Sperm      Live Animals
 Usage Restrictions  The recipient of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. This rat will be made available subject to the following conditions. 1. The rat is to be used either by a)scientists affiliated with a non-profit research institution who are engaged in academic research. or b)commercial or similar interests, they need a secured written agreement from Nippon Institute for Biological Science (NIBS). 2. The rat never be distributed to third parties. 3. If the rat is referred to in any publication, then correct reference will be made to NIBS. 4. NIBS does not accept any liability whatever in connect with the handling or use of the rat. 
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability   
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others 
  Control Strains   Reporter gene Strains  
 Gene antisense strand for rat Gh1
 Origin The construct which contains of rat growth hormone (<i>Gh1</i>) promoter, 4 copies of thyroid hormone response element (TRE), and antisense sequences for rat <i>Gh1</i> cDNA was injected into Jcl:Wistar embryo (Matsumoto, 1993). This transgenic rat was established at NT Science in 1992, and transferred to Japan Bio Science Laboratory Co., Ltd. in 2003. This strain has been maintained at Nagasaki University Graduate School of Biomedical Science. This strain is one of the Tg lines described as “TRE4 910329-32” in the reference 1 (Matsumoto, 1993). (Jun 21, 2010) 
 Strain Characteristics This rat expresses an antisense RNA for inhibiting <i>Gh1</i> expression in the pituitary and exhibits dwarfism. Plasma GH concentration was lower in both hemizygous and homozygous transgenic rats (Matsumoto, 1995). This transgenic rat can be an animal model for human growth hormone deficiency. (Jun 21, 2010) 
 Breeding Conditions Both hemizygous and homozygous rats have fertility. 
 Genotyping RT-PCR 
 References  Matsumoto K, Kakidani H, Takahashi A, Nakagata N, Anzai M, Matsuzaki Y, Takahashi Y, Miyata K, Utsumi K, Iritani A.
Growth retardation in rats whose growth hormone gene expression was suppressed by antisense RNA transgene.
Mol Reprod Dev. 1993 Sep;36(1):53-8.