Japanese
 NBRP Rat No: 0651 Strain Name: WIAR.MPR-Arsbabd/Ncchd Commmon Name: MPR
 Principal Investigator  XIAO-KANG LI
 Organization   National Research Institute for Child Health and Development Division of Transplantation Immunology
 Address  2-10-1, Okura, Setagaya-ku,

157-8535 Tokyo

  ＪＡＰＡＮ
 Telephone  03-5494-8283  Fax:  03-415-2171   ri-k@ncchd.go.jp
 Inbred Generations    
   
 Coat Color
 Deposition Status
 
 白
  Embryo      Sperm      Live Animals
 Usage Restrictions   
 Genetic Status   Inbred   Segregating   Congenic   Consomic    Recombinant 
  Coisogenic   Spont. Mutant    Transgene   Ind. Mutant    Others 
 Comercial Availability   
 Research Category   Diabetes Obesity    Neurobiology    Ophthalmology    Dentistry    Cardio- Hypertension 
  Oncology   Metabolism   Otorhinology    Immunology    Infectious Disease
  Osteology    Internal Medicine   Dermatology   Reproduction    Development
  Behavior    Hematology    Urology   Pharmacology   Others 
  Control Strains   Reporter gene Strains  
 Gene Arsb: arylsulfatase B
 Origin This congenic strain was established by backcrossing MPR/Iar (provided by Dr. Kunieda, Okayama University) to WIAR/Iar 
 Strain Characteristics This strain does not show organ rejection reaction after organ transplantation.<br>
Under analysis for detailed phenotype.  
 Breeding Conditions Maintained as segregating inbred strain (Arsb is heterozygous). Arsb-homozygous rats (both male and female) are unsuitable for breeding. 
 Genotyping Sequence analysis to detect one-base insertion of C, resulting in right contiguous "C" from nucleotide 664 in the exon3. <br>
Primers: Primer F (GCGAAGAACCTGAAAAGAG) and Primer R (TGCACACACACACACACACA) <br>
PCR condition：80℃ 15min, 94℃ 5min, (94℃0.5min,60℃ 1min,72℃ 1min)&#215;35cycle, 72℃ 7min.<br>
 
 References  Yoshida M, Noguchi J, Ikadai H, Takahashi M, Nagase S.
Arylsulfatase B-deficient mucopolysaccharidosis in rats.
J Clin Invest. 91(3):1099-104, 1993.

Kunieda T, Simonaro CM, Yoshida M, Ikadai H, Levan G, Desnick RJ, Schuchman EH.
Mucopolysaccharidosis type VI in rats: isolation of cDNAs encoding arylsulfatase B, chromosomal localization of the gene, and identification of the mutation.
Genomics. 10;29(3):582-7, 1995.
国枝哲夫,ムコ多糖症Ⅵ型ラットの遺伝子治療のためのモデル動物としての確立,1997

Toyama S, Migita O, Fujino M, Kunieda T, Kosuga M, Fukuhara Y, Nagahara Y, Li XK, Okuyama T.
Liver transplantation: New treatment for mucopolysaccharidosis type VI in rats.
Pediatr Int. 2019 Feb;61(2):180-189. doi: 10.1111/ped.13751. Epub 2019 Feb 21.