ENUミュータジェネシスにより作製されたG1ラット1,735匹の凍結精子が寄託されました。ENU（エチルニトロソウレア）を腹腔内投与したF344/NSlc雄に、F344/NSlc雌を交配して作製されたG1ラットは、ゲノム上に多数の点突然変異を保有しています。
An ENU-induced mutant archive for gene targeting in rats.
Mashimo T. et al. Nat Genet. 2008 May;40(5):514-5.
ENUミュータジェネシスの概要を以下に示します:
ENU is injected intraperitoneally using a split dose of 2 x 40 mg/kg into 9 and 10 weeks old male F344/NSlc rats. These males are mated 10 weeks after the second ENU injection. DNA and sperm of their offspring (G1) are stored at the Kyoto University Rat Mutant Archive (KURMA). The
screening technique MuT-POWER can be applied to the DNA of the archive at any given time in the future and in case of successful mutation detection, intracytoplasmic sperm injection (ICSI) is used to derive viable offspring from the G1 sperms.
Preliminary results suggests that the mutation rate of KURMA is 1 per 3.7 MBp. The current number of G1 animals is 5,000; 10,000 G1 animals will be obtained in the future. An excerpt of some mutant genes with successful screening outcome is listed below.