Yoshimi Lab | Publications

Publications

論文
2026	CRISPR-Cas3-based editing for targeted deletions in a mouse model of transthyretin amyloidosis. Ishida S, Sato Y, Chosa K, Ezawa E, Yamauchi Y, Oyama M, Kozuka-Hata H, Ito R, Sato R, Maeki M, Ishikawa TO, Yamamura K, Takeshita K, Yamaguchi K, Kochi Y, Hashiya F, Liu Y, Abe N, Abe H, Sekijima Y, Yoshimi K, Mashimo T. Nat Biotechnol. 2026 Jan 5. doi: 10.1038/s41587-025-02949-6.
	COXFA4L3 enhances mitochondrial complex IV function to boost ATP synthesis and drive sperm motility. Tokito R, Oishi K, Sugiyama T, Fujisawa Y, Kuba F, Yoshida K, Yoshida K, Yoshida M, Tanaka Y, Amo T, Yamaguchi N, Akiyama T, Imai Y, Yoshimi K, Koide T, Kurihara Y. Mitochondrion. 2026 Jan;86:102082.
2025	Versatile Genome Editing Using Type I-E CRISPR-Cas3 in Rice. Saika H, Hara N, Yasumoto S, Muranaka T, Yoshimi K, Mashimo T, Toki S. Plant Cell Physiol. 2025 Oct 28:pcaf138.
	Diverse Cre recombinase expression pattern in Albumin-Cre driver rats. Ishida S, Taguchi K, Iida R, Hattori K, Taketsuru H, Yoshimi K, Yamamoto M, Mashimo T. Exp Anim. 2025 Jul 11;74(3):328-334.
	Sustainable and portable CRISPR-based diagnostics for high-sensitivity Mpox detection. Hirano R, Yoshimi K, Asano K, Takeshita K, Ishii JK, Sato K, Mashimo T. NPJ Biosens*. 2025; 2, 43.
2024	CRISPR Diagnostics for Quantification and Rapid Diagnosis of Myotonic Dystrophy Type 1 Repeat Expansion Disorders. Asano K, Yoshimi K, Takeshita K, Mitsuhashi S, Kochi Y, Hirano R, Tingyu Z, Ishida S, Mashimo T. ACS Synth Biol*. 2024 Dec 20;13(12):3926-3935.
	Genome editing using type I-E CRISPR-Cas3 in mice and rat zygotes. Yoshimi K, Kuno A, Yamauchi Y, Hattori K, Taniguchi H, Mikamo K, Iida R, Ishida S, Goto M, Takeshita K, Ito R, Takahashi R, Takahashi S, Mashimo T. Cell Rep Methods*. 2024 Aug 19;4(8):100833.
	A novel Kit mutant rat enables hematopoietic stem cell engraftment without irradiation. Iida R, Ishida S, Wang J, Hattori K, Yoshimi K, Yamazaki S, Mashimo T. Exp Hematol. 2024 Apr;132:104174.
2023	Cyba and Nox2 mutant rats show different incidences of eosinophilia in the genetic background- and sex-dependent manner. Mori M, Dai J, Miyahara H, Li Y, Kang X, Yoshimi K, Mashimo T, Higuchi K, Matsumoto K. Exp Anim. 2023 May 17;72(2):233-241.
2022	A heterozygous LAMA5 variant may contribute to slowly progressive, vinculin-enhanced familial FSGS and pulmonary defects. Kaimori JY, Kikkawa Y, Motooka D, Namba-Hamano T, Takuwa A, Okazaki A, Kobayashi K, Tanigawa A, Kotani Y, Uno Y, Yoshimi K, Hattori K, Asahina Y, Kajimoto S, Doi Y, Oka T, Sakaguchi Y, Mashimo T, Sekiguchi K, Nakaya A, Nomizu M, Isaka Y. JCI Insight. 2022 Dec 8;7(23):e158378.
	Dynamic mechanisms of CRISPR interference by Escherichia coli CRISPR-Cas3. Yoshimi K, Takeshita K, Kodera N, Shibumura S, Yamauchi Y, Omatsu M, Umeda K, Kunihiro Y, Yamamoto M, Mashimo T. Nat Commun*. 2022 Aug 30;13(1):4917.
	A high-quality severe combined immunodeficiency (SCID) rat bioresource. Miyasaka Y, Wang J, Hattori K, Yamauchi Y, Hoshi M, Yoshimi K, Ishida S, Mashimo T. PLoS One. 2022 Aug 12;17(8):e0272950.
	The role of cell-autonomous circadian oscillation of Cry transcription in circadian rhythm generation. Matsumura R, Yoshimi K, Sawai Y, Yasumune N, Kajihara K, Maejima T, Koide T, Node K, Akashi M. Cell Rep. 2022 Apr 19;39(3):110703.
	Study of the CRISPR/Cas3 System for Xenotransplantation. Miyagawa S, Watanabe M, Nagashima H, Sato K, Kogata S, Toyama C, Masahata K, Kamiyama M, Yamamoto R, Eguchi H, Maeda A, Yoshimi K, Mashimo T, Okuyama H. Transplant Proc. 2022 Mar;54(2):522-524.
	CRISPR-Cas3-based diagnostics for SARS-CoV-2 and influenza virus. Yoshimi K* Takeshita K, Yamayoshi S, Shibumura S, Yamauchi Y, Yamamoto M, Yotsuyanagi H, Kawaoka Y, Mashimo T. iScience. 2022 Feb 18;25(2):103830.
2021	Next-generation prebiotic promotes selective growth of bifidobacteria, suppressing Clostridioides difficile. Hirano R, Sakanaka M, Yoshimi K, Sugimoto N, Eguchi S, Yamauchi Y, Nara M, Maeda S, Ami Y, Gotoh A, Katayama T, Iida N, Kato T, Ohno H, Fukiya S, Yokota A, Nishimoto M, Kitaoka M, Nakai H, Kurihara S. Gut Microbes. 2021 Jan-Dec;13(1):1973835.
	Chemokine ligand 28 (CCL28) negatively regulates trabecular bone mass by suppressing osteoblast and osteoclast activities. Iwamoto R, Takahashi T, Yoshimi K, Imai Y, Koide T, Hara M, Ninomiya T, Nakamura H, Sayama K, Yukita A. J Bone Miner Metab. 2021 Jul;39(4):558-571.
	Combi-CRISPR: combination of NHEJ and HDR provides efficient and precise plasmid-based knock-ins in mice and rats. Yoshimi K, Oka Y, Miyasaka Y, Kotani Y, Yasumura M, Uno Y, Hattori K, Tanigawa A, Sato M, Oya M, Nakamura K, Matsushita N, Kobayashi K, Mashimo T. Hum Genet*. 2021 Feb;140(2):277-287.
	Photoactivatable Cre knock-in mice for spatiotemporal control of genetic engineering in vivo. Yoshimi K, Yamauchi Y, Tanaka T, Shimada T, Sato M, Mashimo T. Lab Invest*. 2021 Jan;101(1):125-135.
2019	CRISPR-Cas3 induces broad and unidirectional genome editing in human cells. Morisaka H, Yoshimi K, Okuzaki Y, Gee P, Kunihiro Y, Sonpho E, Xu H, Sasakawa N, Naito Y, Nakada S, Yamamoto T, Sano S, Hotta A, Takeda J, Mashimo T. Nat Commun*. 2019 Dec 6;10(1):5302.
	Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation. Gurumurthy CB, O'Brien AR, Quadros RM, Adams J Jr, Alcaide P, Ayabe S, Ballard J, Batra SK, Beauchamp MC, Becker KA, Bernas G, Brough D, Carrillo-Salinas F, Chan W, Chen H, Dawson R, DeMambro V, D'Hont J, Dibb KM, Eudy JD, Gan L, Gao J, Gonzales A, Guntur AR, Guo H, Harms DW, Harrington A, Hentges KE, Humphreys N, Imai S, Ishii H, Iwama M, Jonasch E, Karolak M, Keavney B, Khin NC, Konno M, Kotani Y, Kunihiro Y, Lakshmanan I, Larochelle C, Lawrence CB, Li L, Lindner V, Liu XD, Lopez-Castejon G, Loudon A, Lowe J, Jerome-Majewska LA, Matsusaka T, Miura H, Miyasaka Y, Morpurgo B, Motyl K, Nabeshima YI, Nakade K, Nakashiba T, Nakashima K, Obata Y, Ogiwara S, Ouellet M, Oxburgh L, Piltz S, Pinz I, Ponnusamy MP, Ray D, Redder RJ, Rosen CJ, Ross N, Ruhe MT, Ryzhova L, Salvador AM, Alam SS, Sedlacek R, Sharma K, Smith C, Staes K, Starrs L, Sugiyama F, Takahashi S, Tanaka T, Trafford AW, Uno Y, Vanhoutte L, Vanrockeghem F, Willis BJ, Wright CS, Yamauchi Y, Yi X, Yoshimi K, Zhang X, Zhang Y, Ohtsuka M, Das S, Garry DJ, Hochepied T, Thomas P, Parker-Thornburg J, Adamson AD, Yoshiki A, Schmouth JF, Golovko A, Thompson WR, Lloyd KCK, Wood JA, Cowan M, Mashimo T, Mizuno S, Zhu H, Kasparek P, Liaw L, Miano JM, Burgio G. Genome Biol. 2019 Aug 26;20(1):171.
2018	Inhibitory effects of pentoxifylline on inflammation-related tumorigenesis in rat colon. Shirakami Y, Kochi T, Kubota M, Sakai H, Ibuka T, Yoshimi K, Kuramoto T, Tanaka T, Shimizu M, Seishima M. Oncotarget. 2018 Sep 21;9(74):33972-33981.
	CLICK: one-step generation of conditional knockout mice. Miyasaka Y, Uno Y, Yoshimi K, Kunihiro Y, Yoshimura T, Tanaka T, Ishikubo H, Hiraoka Y, Takemoto N, Tanaka T, Ooguchi Y, Skehel P, Aida T, Takeda J, Mashimo T. BMC Genomics. 2018 May 2;19(1):318.
2017	Tremor dominant Kyoto (Trdk) rats carry a missense mutation in the gene encoding the SK2 subunit of small-conductance Ca(2+)-activated K(+) channel. Kuramoto T, Yokoe M, Kunisawa N, Ohashi K, Miyake T, Higuchi Y, Yoshimi K, Mashimo T, Tanaka M, Kuwamura M, Kaneko S, Shimizu S, Serikawa T, Ohno Y. Brain Res. 2017 Dec 1;1676:38-45.
	Hierarchy in the home cage affects behaviour and gene expression in group-housed C57BL/6 male mice. Horii Y, Nagasawa T, Sakakibara H, Takahashi A, Tanave A, Matsumoto Y, Nagayama H, Yoshimi K, Yasuda MT, Shimoi K, Koide T. Sci Rep. 2017 Aug 1;7(1):6991.
2016	ssODN-mediated knock-in with CRISPR-Cas for large genomic regions in zygotes. Yoshimi K, Kunihiro Y, Kaneko T, Nagahora H, Voigt B, Mashimo T. Nat Commun*. 2016 Jan 20;7:10431.
2015	Atopic dermatitis-like skin lesions with IgE hyperproduction and pruritus in KFRS4/Kyo rats. Kuramoto T, Yokoe M, Tanaka D, Yuri A, Nishitani A, Higuchi Y, Yoshimi K, Tanaka M, Kuwamura M, Hiai H, Kabashima K, Serikawa T. J Dermatol Sci. 2015 Nov;80(2):116-23.
	Utility of Apc-mutant rats with a colitis-associated colon carcinogenesis model for chemoprevention studies. Kochi T, Shimizu M, Shirakami Y, Yoshimi K, Kuramoto T, Tanaka T, Moriwaki H. J Cancer Prev. 2015 May;24(3):180-7.
2014	Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR-Cas platform. Yoshimi K, Kaneko T, Voigt B, Mashimo T. Nat Commun*. 2014 Jun 26;5:4240.
2013	Tumor suppressor APC protein is essential in mucosal repair from colonic inflammation through angiogenesis. Yoshimi K, Tanaka T, Serikawa T, Kuramoto T. Am J Pathol*. 2013 Apr;182(4):1263-74.
2012	Use of a chemically induced-colon carcinogenesis-prone Apc-mutant rat in a chemotherapeutic bioassay. Yoshimi K, Hashimoto T, Niwa Y, Hata K, Serikawa T, Tanaka T, Kuramoto T. BMC Cancer*. 2012 Oct 3;12:448.
	Generation and characterization of severe combined immunodeficiency rats. Mashimo T, Takizawa A, Kobayashi J, Kunihiro Y, Yoshimi K, Ishida S, Tanabe K, Yanagi A, Tachibana A, Hirose J, Yomoda J, Morimoto S, Kuramoto T, Voigt B, Watanabe T, Hiai H, Tateno C, Komatsu K, Serikawa T. Cell Rep. 2012 Sep 27;2(3):685-94.
2010	Generation of knockout rats with X-linked severe combined immunodeficiency (X-SCID) using zinc-finger nucleases. Mashimo T, Takizawa A, Voigt B, Yoshimi K, Hiai H, Kuramoto T, Serikawa T. PLoS One. 2010 Jan 25;5(1):e8870.
2009	Enhanced colitis-associated colon carcinogenesis in a novel Apc mutant rat. Yoshimi K, Tanaka T, Takizawa A, Kato M, Hirabayashi M, Mashimo T, Serikawa T, Kuramoto T. Cancer Sci*. 2009 Nov;100(11):2022-7.

総説
2022	Genome editing technology and applications with the type I CRISPR system. Yoshimi K, Mashimo T. Gene and Genome Editing*. 2022 Dec 30;3-4: 100013.
2018	Application of genome editing technologies in rats for human disease models. Yoshimi K, Mashimo T. J Hum Genet*. 2018 Feb;63(2):115-123.
2014	The utility of Apc-mutant rats in modeling human colon cancer. Irving AA, Yoshimi K, Hart ML, Parker T, Clipson L, Ford MR, Kuramoto T, Dove WF, Amos-Landgraf JM. Dis Model Mech. 2014 Nov;7(11):1215-25.