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Rat Strains (FOB: Hand-held Observations)
Hand-held observations
(Observe animals' reactions when they are removed from their cage and held.)
1) Reactivity: Choose the item from the following list that best describes the ease with which the animal can be removed from its cage (scored).
1: Very easy (animal sits and allows itself to be picked up)
2: Easy/normal (animal does not resist)
3: Somewhat difficult (rat stands)
4: Difficult (animal cringes and becomes rigid or runs around and is difficult to grab)
5: Very difficult (animal attacks)
2) Handling: Choose the item from the following list that best describes the ease with which the animal can be handled after having been removed from cage (scored).
1: Does not resist/ very easy to handle
2: Squeaks or does not squeak but exhibits mild resistance; easy to handle/normal
3: Animal freezes and does not move; becomes rigid in hand
4: Very difficult (animal struggles, squirms, appears distressed, attempts to bite)
3) Palpebral closure: Choose the item from the following list that best describes the closure of the animal's eyes when held in the hand (scored).
1: None/normal/ (eyes are open)
2: Slightly sagging/half shut (eyelids slightly lowered)
3: Shut (eyelids are closed)
4) Lacrimation: Holding the rat, observe areas around eyes and choose the item from the following list that best describes the animal's condition (scored).
1: No lacrimation/normal
2: Moisture only around eyes
3: Moisture flows from eyes
5) Salivation: Holding the rat, observe areas around mouth and choose the item from the following list that best describes the animal's condition (scored).
1: No salivation/normal
2: Moisture only around mouth
3: Moisture flows from mouth
6) Piloerection: Holding the rat, observe coat (for degree of piloerection) and choose the item from the following list that best describes the animal's condition (scored).
1: None/normal
2: Around head and back (mild piloerection only around head and back)
3: Generalized (piloerection all over body)
7) Others (to be recorded as description): Note in detail any findings related to dirtiness of hair cast, bite marks, missing nails, gauntness (stomach can be touched, median vertebrae protrude) or death (findings).
Enter a minus sign (-) in the findings column if no such behavior is observed.