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Rat Strains (FOB: Hand-held Observations) 
 
Strainname 		  
Reactivity 		  
Handling 
Palpebral Closure Hand  		  
Lacrimation 		  
Salivation		  
Piloerection		  
Others 
ACI/NHok  2,2,2,2,2,2   2,2,2,2,2,2   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   -,-,-,-,-,- 
ACI/NJcl  2,2,2,2,2,2   2,2,2,2,2,2   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   -,-,-,-,-,- 
ACI/NKyo  2,2,2,3,2,2   2,2,2,2,2,2   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   -,-,-,-,-,- 
ACI/Nkyo(F)  2,2,2,2,2,2   2,2,2,2,2,2   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   -,-,-,-,-,- 
ACI/NMna  2,2,2,2,2,2   2,2,2,2,2,2   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   -,-,-,-,-,- 
ACI/NSlc  2,2,2,2,2,2   2,2,2,2,2,2   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   -,-,-,-,-,- 
ACI-Lystbg-Kyo/Kyo  2,2,2,2,2,2   2,2,2,2,2,2   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   -,-,-,-,-,- 
ALB/Hok(F)  2,2,2,2,2,2   2,2,2,2,2,2   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   -,-,-,-,-,- 
BN/1Hok  2,2,2,2,2,2   2,2,2,2,2,2   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   -,-,-,-,-,- 
BN/fMaiHok  2,2,2,2,2,2   2,2,2,2,2,2   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   -,-,-,-,-,- 
BN/KtsSlc  2,2,2,2,2,2   2,2,2,2,2,2   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   -,-,-,-,-,- 
BN/KunKtsSlc  2,2,2,2,2,2   2,2,2,2,2,2   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   -,-,-,-,-,- 
BN/Seac  2,2,2,2,2,2   2,1,2,1,2,1   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   -,-,-,+,-,- 
BN/SsNHsd  2,2,2,2,2,2   2,2,2,2,2,2   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   -,-,-,-,-,- 
BN/SsNSlc  2,2,2,2,2,2   2,2,2,2,2,2   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   -,-,-,-,-,- 
BUF/Mna  2,2,2,2,2,2   2,2,2,2,2,2   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   -,-,-,-,-,- 
DA/Slc  2,2,2,2,2,2   2,2,2,2,2,2   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   -,-,-,-,-,- 
Dahl S/JrSeac  2,2,2,2,2,2   2,2,2,2,2,2   2,1,1,1,1,1   2,1,1,1,1,1   2,1,1,1,1,1   2,1,1,1,1,1   -,-,-,-,-,- 
DDI/Ddia  2,2,2,2,2,2   2,2,2,2,2,2   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   -,-,-,-,-,- 
DMY/Kyo  2,2,2,2,2,2   2,2,2,2,2,2   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   1,1,1,1,1,1   -,-,-,-,-,- 
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Hand-held observations
(Observe animals' reactions when they are removed from their cage and held.)

1) Reactivity: Choose the item from the following list that best describes the ease with which the animal can be removed from its cage (scored).


1: Very easy (animal sits and allows itself to be picked up)
2: Easy/normal (animal does not resist)
3: Somewhat difficult (rat stands)
4: Difficult (animal cringes and becomes rigid or runs around and is difficult to grab)
5: Very difficult (animal attacks)


2) Handling: Choose the item from the following list that best describes the ease with which the animal can be handled after having been removed from cage (scored).


1: Does not resist/ very easy to handle
2: Squeaks or does not squeak but exhibits mild resistance; easy to handle/normal
3: Animal freezes and does not move; becomes rigid in hand
4: Very difficult (animal struggles, squirms, appears distressed, attempts to bite)


3) Palpebral closure: Choose the item from the following list that best describes the closure of the animal's eyes when held in the hand (scored).


1: None/normal/ (eyes are open)
2: Slightly sagging/half shut (eyelids slightly lowered)
3: Shut (eyelids are closed)


4) Lacrimation: Holding the rat, observe areas around eyes and choose the item from the following list that best describes the animal's condition (scored).


1: No lacrimation/normal
2: Moisture only around eyes
3: Moisture flows from eyes


5) Salivation: Holding the rat, observe areas around mouth and choose the item from the following list that best describes the animal's condition (scored).


1: No salivation/normal
2: Moisture only around mouth
3: Moisture flows from mouth


6) Piloerection: Holding the rat, observe coat (for degree of piloerection) and choose the item from the following list that best describes the animal's condition (scored).


1: None/normal
2: Around head and back (mild piloerection only around head and back)
3: Generalized (piloerection all over body)


7) Others (to be recorded as description): Note in detail any findings related to dirtiness of hair cast, bite marks, missing nails, gauntness (stomach can be touched, median vertebrae protrude) or death (findings).
Enter a minus sign (-) in the findings column if no such behavior is observed.